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Simultaneous cell disruption and aqueous two-phase extraction for isolation of intracellular recombinant proteins
Alternative TitleChin. J. Chem. Eng.
Wang, GH; Feng, XL; Su, ZG
1999-06-01
Source PublicationCHINESE JOURNAL OF CHEMICAL ENGINEERING
ISSN1004-9541
Volume7Issue:2Pages:139-144
AbstractA new technique was developed for the integrated processing of cell disruption and aqueous two-phase extraction in a high-speed bead mill to separate intracellular proteins from microbial cells. The process was named as simultaneous cell disruption and aqueous two-phase extraction (SDATE). Advantages, such as high cell disruption efficiency, biochemical activities preservation of proteins, cell debris elimination, and preliminary purification of the target protein were being claimed. When this technique was employed for isolating recombinant Tumor Necrosis Factor (TNF) from E. coli, overall protein concentration and TNF activity were found to have been increased. More than 95% of TNF was partitioned into the top phase and all cell debris were in the bottom phase. The partition coefficient was greater than 3 and the TNF purification factor was greater than 6. It is shown that less separation steps were being utilized in the new technique, meaning a reduction in separation time and less process extractors required.; A new technique was developed for the integrated processing of cell disruption and aqueous two-phase extraction in a high-speed bead mill to separate intracellular proteins from microbial cells. The process was named as simultaneous cell disruption and aqueous two-phase extraction (SDATE). Advantages, such as high cell disruption efficiency, biochemical activities preservation of proteins, cell debris elimination, and preliminary purification of the target protein were being claimed. When this technique was employed for isolating recombinant Tumor Necrosis Factor (TNF) from E. coli, overall protein concentration and TNF activity were found to have been increased. More than 95% of TNF was partitioned into the top phase and all cell debris were in the bottom phase. The partition coefficient was greater than 3 and the TNF purification factor was greater than 6. It is shown that less separation steps were being utilized in the new technique, meaning a reduction in separation time and less process extractors required.
KeywordSdate Release Of Intracellular Proteins High Speed Bead Mill Aqueous Two-phase Extraction Tumor Necrosis Factor
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordTUMOR NECROSIS FACTOR ; PURIFICATION
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
WOS IDWOS:000081096200007
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6064
Collection研究所(批量导入)
Affiliation1.Dalian Univ Technol, Biochem Engn Lab, Dalian 116012, Peoples R China
2.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Wang, GH,Feng, XL,Su, ZG. Simultaneous cell disruption and aqueous two-phase extraction for isolation of intracellular recombinant proteins[J]. CHINESE JOURNAL OF CHEMICAL ENGINEERING,1999,7(2):139-144.
APA Wang, GH,Feng, XL,&Su, ZG.(1999).Simultaneous cell disruption and aqueous two-phase extraction for isolation of intracellular recombinant proteins.CHINESE JOURNAL OF CHEMICAL ENGINEERING,7(2),139-144.
MLA Wang, GH,et al."Simultaneous cell disruption and aqueous two-phase extraction for isolation of intracellular recombinant proteins".CHINESE JOURNAL OF CHEMICAL ENGINEERING 7.2(1999):139-144.
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