Knowledge Management System Of Institute of process engineering,CAS
| Spectral study of the microenvironment change of aromatic amino-acid residues in BSA induced by pH | |
| Alternative Title | Spectrosc. Spectr. Anal. |
| Wei, XF; Ding, XM; Liu, HZ | |
| 2000-08-01 | |
| Source Publication | SPECTROSCOPY AND SPECTRAL ANALYSIS
 |
| ISSN | 1000-0593 |
| Volume | 20Issue:4Pages:556-559 |
| Abstract | The microenvironment change of aromatic amino-acid residues in BSA at various pH were studied by fluorescence spectra and ultraviolet absorption spectra. It suggested that the change of protein surface and surface hydrophobicity from result. Compared to neutral pH, the Trp microenvironment seemed to be more hydrophobic at lower pH indicating less local surface hydrophobicity of BSA. At higher pH, the protein undergo conformational changes and undold, the more buried Phe exposed to solvents consistent with stronger surface hydrophobicity of BSA.; The microenvironment change of aromatic amino-acid residues in BSA at various pH were studied by fluorescence spectra and ultraviolet absorption spectra. It suggested that the change of protein surface and surface hydrophobicity from result. Compared to neutral pH, the Trp microenvironment seemed to be more hydrophobic at lower pH indicating less local surface hydrophobicity of BSA. At higher pH, the protein undergo conformational changes and undold, the more buried Phe exposed to solvents consistent with stronger surface hydrophobicity of BSA. |
| Keyword | Bovine Serum Albumin Conformation Microenvironment Surface Hydrophobicity |
| Subtype | Article |
| WOS Headings | Science & Technology ; Technology |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Spectroscopy |
| WOS Subject | Spectroscopy |
| WOS ID | WOS:000089130900028 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/6073 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.Chinese Acad Sci, Inst Chem Met, Young Scientist Lab Separat Sci & Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Inst Photog Chem, Beijing 100021, Peoples R China |
| Recommended Citation GB/T 7714 | Wei, XF,Ding, XM,Liu, HZ. Spectral study of the microenvironment change of aromatic amino-acid residues in BSA induced by pH[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2000,20(4):556-559. |
| APA | Wei, XF,Ding, XM,&Liu, HZ.(2000).Spectral study of the microenvironment change of aromatic amino-acid residues in BSA induced by pH.SPECTROSCOPY AND SPECTRAL ANALYSIS,20(4),556-559. |
| MLA | Wei, XF,et al."Spectral study of the microenvironment change of aromatic amino-acid residues in BSA induced by pH".SPECTROSCOPY AND SPECTRAL ANALYSIS 20.4(2000):556-559. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| pH诱导牛血清白蛋白芳香氨基酸残基微环境（146KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
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