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Spectral study of the microenvironment change of aromatic amino-acid residues in BSA induced by pH
Alternative TitleSpectrosc. Spectr. Anal.
Wei, XF; Ding, XM; Liu, HZ
2000-08-01
Source PublicationSPECTROSCOPY AND SPECTRAL ANALYSIS
ISSN1000-0593
Volume20Issue:4Pages:556-559
AbstractThe microenvironment change of aromatic amino-acid residues in BSA at various pH were studied by fluorescence spectra and ultraviolet absorption spectra. It suggested that the change of protein surface and surface hydrophobicity from result. Compared to neutral pH, the Trp microenvironment seemed to be more hydrophobic at lower pH indicating less local surface hydrophobicity of BSA. At higher pH, the protein undergo conformational changes and undold, the more buried Phe exposed to solvents consistent with stronger surface hydrophobicity of BSA.; The microenvironment change of aromatic amino-acid residues in BSA at various pH were studied by fluorescence spectra and ultraviolet absorption spectra. It suggested that the change of protein surface and surface hydrophobicity from result. Compared to neutral pH, the Trp microenvironment seemed to be more hydrophobic at lower pH indicating less local surface hydrophobicity of BSA. At higher pH, the protein undergo conformational changes and undold, the more buried Phe exposed to solvents consistent with stronger surface hydrophobicity of BSA.
KeywordBovine Serum Albumin Conformation Microenvironment Surface Hydrophobicity
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
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Indexed BySCI
Language英语
WOS Research AreaSpectroscopy
WOS SubjectSpectroscopy
WOS IDWOS:000089130900028
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Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6073
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Chem Met, Young Scientist Lab Separat Sci & Engn, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Inst Photog Chem, Beijing 100021, Peoples R China
Recommended Citation
GB/T 7714
Wei, XF,Ding, XM,Liu, HZ. Spectral study of the microenvironment change of aromatic amino-acid residues in BSA induced by pH[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2000,20(4):556-559.
APA Wei, XF,Ding, XM,&Liu, HZ.(2000).Spectral study of the microenvironment change of aromatic amino-acid residues in BSA induced by pH.SPECTROSCOPY AND SPECTRAL ANALYSIS,20(4),556-559.
MLA Wei, XF,et al."Spectral study of the microenvironment change of aromatic amino-acid residues in BSA induced by pH".SPECTROSCOPY AND SPECTRAL ANALYSIS 20.4(2000):556-559.
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