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Suspension culture of compact callus aggregate of Rhodiola sachalinensis for improved salidroside production
Alternative TitleEnzyme Microb. Technol.
Xu, JF; Su, ZG; Feng, PS
1998-07-01
Source PublicationENZYME AND MICROBIAL TECHNOLOGY
ISSN0141-0229
Volume23Issue:1-2Pages:20-27
AbstractSome level of cellular or tissue differentiation is needed for the biosynthesis of many important compounds in plant cell cultures. The culture of compact callus aggregates (CCA) consisting of spherical, smooth-surfaced clumps of 2-7 mm in diameter displaying some level of cellular and tissue differentiation was initiated from the stems of R. sachalinensis. Histochemical analysis showed that the CCA were structurally organized to facilitate nutrient transport. The kinetics for CCA growth and salidroside synthesis were measured in shake flasks. it was found that a short phase of exponential growth (mu = 0.137 day(-1)) and a relatively long phase of linear growth (growth rate = 0.60 g day(-1) l(-1) predominated over most of the the culture cycle. The salidroside yield of CCA after 24 days of culture was 0.68% on a dry weight basis, approximately six times as much as that of the dispersed cells; however, the growth rate of CCA was comparable to that of the dispersed cells. The CCA suspension culture was also successfully carried out ill a 5-l airlift bioreactor. The yield of salidroside was similar to that of shake-flask cultures though the biomass was relatively small. No foam buildup occurred in the reactor Since rite medium remained almost clear throughout the culture cycle. (C) 1998 Elsevier Science Inc.; Some level of cellular or tissue differentiation is needed for the biosynthesis of many important compounds in plant cell cultures. The culture of compact callus aggregates (CCA) consisting of spherical, smooth-surfaced clumps of 2-7 mm in diameter displaying some level of cellular and tissue differentiation was initiated from the stems of R. sachalinensis. Histochemical analysis showed that the CCA were structurally organized to facilitate nutrient transport. The kinetics for CCA growth and salidroside synthesis were measured in shake flasks. it was found that a short phase of exponential growth (mu = 0.137 day(-1)) and a relatively long phase of linear growth (growth rate = 0.60 g day(-1) l(-1) predominated over most of the the culture cycle. The salidroside yield of CCA after 24 days of culture was 0.68% on a dry weight basis, approximately six times as much as that of the dispersed cells; however, the growth rate of CCA was comparable to that of the dispersed cells. The CCA suspension culture was also successfully carried out ill a 5-l airlift bioreactor. The yield of salidroside was similar to that of shake-flask cultures though the biomass was relatively small. No foam buildup occurred in the reactor Since rite medium remained almost clear throughout the culture cycle. (C) 1998 Elsevier Science Inc.
KeywordRhodiola Sachalinensis Salidroside Suspension Culture Plant Cell Aggregate
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPLANT-CELL CULTURES ; FED-BATCH CULTURE ; HAIRY ROOT ; GROWTH
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000074839200004
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6088
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Dalian Univ Technol, Inst Biochem Engn, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Xu, JF,Su, ZG,Feng, PS. Suspension culture of compact callus aggregate of Rhodiola sachalinensis for improved salidroside production[J]. ENZYME AND MICROBIAL TECHNOLOGY,1998,23(1-2):20-27.
APA Xu, JF,Su, ZG,&Feng, PS.(1998).Suspension culture of compact callus aggregate of Rhodiola sachalinensis for improved salidroside production.ENZYME AND MICROBIAL TECHNOLOGY,23(1-2),20-27.
MLA Xu, JF,et al."Suspension culture of compact callus aggregate of Rhodiola sachalinensis for improved salidroside production".ENZYME AND MICROBIAL TECHNOLOGY 23.1-2(1998):20-27.
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