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Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase highspeed counter-current chromatography
Liu, Dan2; Su, Zhiguo1; Wang, Changhai2; Gu, Ming1; Xing, Siliang3; Gu, M
2010-08-01
Source PublicationJOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
Volume33Issue:15Pages:2266-2271
AbstractThree hydrolyzable tannins, geraniin, corilagin and gallic acid, main active components of Geranium wilfordii Maxim, have been separated and purified in one-step by both reversed-phase and normal-phase high-speed counter-current chromatography. Gallic acid, corilagin and geraniin were purified from 70% aqueous acetone extract of G. wilfordii Maxim with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (1:10:0.2:0.2:20) by reversed-phase high-speed counter-current chromatography at purities of 94.2, 91.0 and 91.3%, at yields of 89.3, 82.9 and 91.7%, respectively. Gallic acid, corilagin and geraniin were purified with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (0.2:10:2:1:5) by normal-phase high-speed counter-current chromatography at purities of 85.9, 92.2 and 87.6%, at yields of 87.4, 94.6 and 94.3%, respectively. It was successful for both reversed-phase and normal-phase high-speed counter-current chromatography to separate high-polarity of low-molecular-weight substances.
KeywordCorilagin Gallic Acid Geraniin Normal-phase High-speed Counter-current Chromatography Reversed-phase High-speed Counter-current Chromatography
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1002/jssc.200900599
Indexed BySCI
Language英语
WOS KeywordPHYLLANTHUS-URINARIA ; PARTITION CHROMATOGRAPHY ; PREPARATIVE ISOLATION ; GALLIC ACID ; BUNGE
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000281055900006
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6110
Collection生化工程国家重点实验室
Corresponding AuthorGu, M
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian, Peoples R China
3.Beijing Kangmingyousheng Biochem Technol Co Ltd, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Liu, Dan,Su, Zhiguo,Wang, Changhai,et al. Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase highspeed counter-current chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(15):2266-2271.
APA Liu, Dan,Su, Zhiguo,Wang, Changhai,Gu, Ming,Xing, Siliang,&Gu, M.(2010).Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase highspeed counter-current chromatography.JOURNAL OF SEPARATION SCIENCE,33(15),2266-2271.
MLA Liu, Dan,et al."Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase highspeed counter-current chromatography".JOURNAL OF SEPARATION SCIENCE 33.15(2010):2266-2271.
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