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A beta-xylosidase from cell wall of maize: Purification, properties and its use in hydrolysis of plant cell wall
Han, Yejun; Chen, Hongzhang; Chen, HZ
2010-05-01
Source PublicationJOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
ISSN1381-1177
Volume63Issue:3-4Pages:135-140
AbstractTo study the potential of plant glycoside hydrolase for hemicelluloses hydrolysis, a beta-xylosidase with molecular weight of 68.5 kDa was purified from the maize during senescent stage. The optimal conditions for the beta-xylosidase were 37 degrees C and pH 4.5. In absence of substrate, the beta-xylosidase was comparatively stable at 37 degrees C and pH 4.5-5.5. At the optimum condition, the K(m) and k(cat) values of the beta-xylosidase against p-nitrophenyl-xyloside were 2.5 mM and 6.5 s(-1), respectively. The enzyme activity was promoted by LiCl, CaCl(2), MnCl(2), MgCl(2), KCl. and NaCl, however severely inhibited by CuCl(2), ZnCl(2), AgNO(3), HgCl(2), and NiCl(2). The purified beta-xylosidase was active against xylobiose, xylotriose, xylotetraose, and xylopentaose. In hydrolysis of corn stover hemicellulose, the xylose production increased by 94.9% and 140% when Trichoderma reesei hemicellulase supplemented with purified beta-xylosidase and crude cell wall proteins of corn stover, respectively. The biochemical characterization of the maize beta-xylosidase makes it a promising candidate enzyme additive for hemicelluloses hydrolysis. (C) 2010 Elsevier By. All rights reserved.
KeywordBeta-xylosidase Plant Cell Wall Proteins Hemicellulose Enzymatic Hydrolysis
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.molcatb.2010.01.004
Indexed BySCI
Language英语
WOS KeywordMOLECULAR-BIOLOGY ; PROTEINS ; EXTRACTION ; XYLANASE ; TRANSXYLOSYLATION ; EXPRESSION
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Physical
WOS IDWOS:000276116900005
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6158
Collection生化工程国家重点实验室
Corresponding AuthorChen, HZ
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Han, Yejun,Chen, Hongzhang,Chen, HZ. A beta-xylosidase from cell wall of maize: Purification, properties and its use in hydrolysis of plant cell wall[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2010,63(3-4):135-140.
APA Han, Yejun,Chen, Hongzhang,&Chen, HZ.(2010).A beta-xylosidase from cell wall of maize: Purification, properties and its use in hydrolysis of plant cell wall.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,63(3-4),135-140.
MLA Han, Yejun,et al."A beta-xylosidase from cell wall of maize: Purification, properties and its use in hydrolysis of plant cell wall".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 63.3-4(2010):135-140.
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