CAS OpenIR  > 生化工程国家重点实验室
A novel biotransformation process of 4'-demethylepipodophyllotoxin to 4'-demethylepipodophyllic acid by Bacillus fusiformis CICC 20463, Part II: process optimization
Tang, Ya-Jie1,2; Xu, Xiao-Ling1; Zhong, Jian-Jiang3; Tang, YJ
2010-02-01
Source PublicationBIOPROCESS AND BIOSYSTEMS ENGINEERING
ISSN1615-7591
Volume33Issue:2Pages:237-246
AbstractThis work optimized the novel biotransformation process of 4'-demethylepipodophyllotoxin (DMEP) into 4'-demethylepipodophyllic acid (DMEPA) by Bacillus fusiformis CICC 20463. Firstly, the biotransformation process was significantly affected by medium composition. 5 g/L of yeast extract and 10 g/L of peptone were optimal for DMEPA production (i.e., 2.81 +/- A 0.21 mg/L), while not beneficial for the cell growth of B. fusiformis. This indicated that the biosynthesis of DMEPA was not corresponded well to the cell growth of B. fusiformis. 40 g/L of sucrose was optimal for DMEPA production (i.e., 2.94 +/- A 0.17 mg/L), and 3 g/L of NaCl was the best for DMEPA production (i.e., 4.10 +/- A 0.18 mg/L). Secondly, the production of DMEPA was significantly enhanced by the control of substrate concentration and culture pH. 100 mg/L of substrate was optimal for DMEPA production (i.e., 6.47 +/- A 0.35 mg/L), and DMEPA concentration was enhanced to 38.78 mg/L by controlling culture pH at 9.0 in the stirred-tank bioreactors. The fundamental information obtained in this study provides a simple and efficient way to produce DMEPA by biotransformation.
KeywordBiotransformation Process Optimization 4 '-demethylepipodophyllotoxin Bacillus Fusiformis 4 '-demethylepipodophyllic Acid
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1007/s00449-009-0317-x
Indexed BySCI
Language英语
WOS KeywordCELL-SUSPENSION CULTURES ; PODOPHYLLOTOXIN CONGENERS ; BIOLOGICAL EVALUATION ; ANALOGS ; 4'-DEMETHYLEPIPODOPHYLLOTOXIN ; PICROPODOPHYLLIN ; BIOCATALYSIS ; DERIVATIVES ; LIGNANS ; AGENTS
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000273588200007
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6214
Collection生化工程国家重点实验室
Corresponding AuthorTang, YJ
Affiliation1.Hubei Univ Technol, Coll Bioengn, Minist Educ, Key Lab Fermentat Engn,Hubei Prov Key Lab Ind Mic, Wuhan 430068, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
3.Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Minist Educ, Key Lab Microbial Metab, Shanghai 200240, Peoples R China
Recommended Citation
GB/T 7714
Tang, Ya-Jie,Xu, Xiao-Ling,Zhong, Jian-Jiang,et al. A novel biotransformation process of 4'-demethylepipodophyllotoxin to 4'-demethylepipodophyllic acid by Bacillus fusiformis CICC 20463, Part II: process optimization[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2010,33(2):237-246.
APA Tang, Ya-Jie,Xu, Xiao-Ling,Zhong, Jian-Jiang,&Tang, YJ.(2010).A novel biotransformation process of 4'-demethylepipodophyllotoxin to 4'-demethylepipodophyllic acid by Bacillus fusiformis CICC 20463, Part II: process optimization.BIOPROCESS AND BIOSYSTEMS ENGINEERING,33(2),237-246.
MLA Tang, Ya-Jie,et al."A novel biotransformation process of 4'-demethylepipodophyllotoxin to 4'-demethylepipodophyllic acid by Bacillus fusiformis CICC 20463, Part II: process optimization".BIOPROCESS AND BIOSYSTEMS ENGINEERING 33.2(2010):237-246.
Files in This Item:
File Name/Size DocType Version Access License
20131127-1325-复件 art(462KB) 限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Tang, Ya-Jie]'s Articles
[Xu, Xiao-Ling]'s Articles
[Zhong, Jian-Jiang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Tang, Ya-Jie]'s Articles
[Xu, Xiao-Ling]'s Articles
[Zhong, Jian-Jiang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Tang, Ya-Jie]'s Articles
[Xu, Xiao-Ling]'s Articles
[Zhong, Jian-Jiang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.