| Synergism between hydrophobic proteins of corn stover and cellulase in lignocellulose hydrolysis | |
Han, YeJun; Chen, HongZhang ; Chen, HZ
| |
| 2010-01-15 | |
| Source Publication | BIOCHEMICAL ENGINEERING JOURNAL
 |
| ISSN | 1369-703X |
| Volume | 48Issue:2Pages:218-224 |
| Abstract | To investigate the characteristics and Values of plant proteins for lignocellulose conversion, cellulase synergetic proteins for lignocellulose hydrolysis were separated from fresh corn stover. The synergetic proteins have strong hydrophobic characterization, yet no cellulose hydrolytic activity. Mass spectrometry analysis indicated that the hydrophobic protein with highest abundance might be cell membrane protein of Zea mays. Compared with steam-exploded corn stover hydrolysis with microbial cellulase mixture, the glucose production was improved by 31.88% with hydrophobic proteins addition. Synergetic mechanism analysis indicated that the hydrophobic proteins reduced unspecific cellulase adsorption onto non-cellulose component of lignocelluloses during hydrolysis. The effect is more significant for exoglucanase and beta-glucosidase. The amount of hydrophobic proteins in fresh corn stover is large. Therefore, Supplementing microbial cellulase complex with hydrophobic proteins of plant for lignocellulose hydrolysis is a promising way for cost reduction in lignocellulose conversion. (C) 2009 Elsevier B.V. All Fights reserved. |
| Keyword | Cellulase Hydrophobic Proteins Corn Stover Synergism Lignocellulose Enzymatic Hydrolysis |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| DOI | 10.1016/j.bej.2009.10.013 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | ENZYMATIC-HYDROLYSIS ; ETHANOL ; STRAW |
| WOS Research Area | Biotechnology & Applied Microbiology ; Engineering |
| WOS Subject | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| WOS ID | WOS:000274350000011 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/6225 |
| Collection | 生化工程国家重点实验室 |
| Corresponding Author | Chen, HZ |
| Affiliation | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| Recommended Citation GB/T 7714 | Han, YeJun,Chen, HongZhang,Chen, HZ. Synergism between hydrophobic proteins of corn stover and cellulase in lignocellulose hydrolysis[J]. BIOCHEMICAL ENGINEERING JOURNAL,2010,48(2):218-224. |
| APA | Han, YeJun,Chen, HongZhang,&Chen, HZ.(2010).Synergism between hydrophobic proteins of corn stover and cellulase in lignocellulose hydrolysis.BIOCHEMICAL ENGINEERING JOURNAL,48(2),218-224. |
| MLA | Han, YeJun,et al."Synergism between hydrophobic proteins of corn stover and cellulase in lignocellulose hydrolysis".BIOCHEMICAL ENGINEERING JOURNAL 48.2(2010):218-224. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 20131127-1338-复件 1-s（502KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment