CAS OpenIR  > 研究所(批量导入)
Analysis of Gelatin Plasma Substitutes in Blood Based on Detection of Hydroxyproline Derivatives
Alternative TitleArtif. Cells Blood Substit. Biotechnol.
Liu, Tao1,2; Zhang, Guifeng1; Li, Suping1,2; Wang, Yinjue1,2; Ma, Guanghui1; Su, Zhiguo1
2011-02-01
Source PublicationARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
ISSN1073-1199
Volume39Issue:1Pages:19-25
AbstractThe gelatin plasma substitute is often polydisperse and heterogenous, making it difficult to determine the elimination rate and half-life in the body. In this study, one method was developed based on quantitative determination of hydroxyproline derivatives. Two plasma substitutes were prepared by succinylation and genipin-crosslinking, respectively. After transfusion, the blood samples were hydrolyzed and derivatized, and then analyzed by HPLC. A two-phase exponential association equation was used for fitting the time-concentration curves. The results indicated that this method could be used for quantitative determination of gelatin in blood, and the pharmacokinetic parameters such as elimination rate and half-life.; The gelatin plasma substitute is often polydisperse and heterogenous, making it difficult to determine the elimination rate and half-life in the body. In this study, one method was developed based on quantitative determination of hydroxyproline derivatives. Two plasma substitutes were prepared by succinylation and genipin-crosslinking, respectively. After transfusion, the blood samples were hydrolyzed and derivatized, and then analyzed by HPLC. A two-phase exponential association equation was used for fitting the time-concentration curves. The results indicated that this method could be used for quantitative determination of gelatin in blood, and the pharmacokinetic parameters such as elimination rate and half-life.
KeywordGelatin Plasma Substitute Colloid Elimination Rate Half Life High Performance Liquid Chromatography
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.3109/10731199.2010.494582
URL查看原文
Indexed BySCI
Language英语
WOS KeywordLIGHT-SCATTERING ; CAPILLARY-ELECTROPHORESIS ; GENIPIN ; COLLAGEN ; RATS ; QUANTITATION ; METABOLISM ; COLLOIDS ; PROTEIN ; MODEL
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering ; Materials Science
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials
WOS IDWOS:000286938300005
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6267
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Liu, Tao,Zhang, Guifeng,Li, Suping,et al. Analysis of Gelatin Plasma Substitutes in Blood Based on Detection of Hydroxyproline Derivatives[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,2011,39(1):19-25.
APA Liu, Tao,Zhang, Guifeng,Li, Suping,Wang, Yinjue,Ma, Guanghui,&Su, Zhiguo.(2011).Analysis of Gelatin Plasma Substitutes in Blood Based on Detection of Hydroxyproline Derivatives.ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,39(1),19-25.
MLA Liu, Tao,et al."Analysis of Gelatin Plasma Substitutes in Blood Based on Detection of Hydroxyproline Derivatives".ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY 39.1(2011):19-25.
Files in This Item:
File Name/Size DocType Version Access License
ACBSB-2011-39-19-25.(369KB) 限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Tao]'s Articles
[Zhang, Guifeng]'s Articles
[Li, Suping]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Tao]'s Articles
[Zhang, Guifeng]'s Articles
[Li, Suping]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Tao]'s Articles
[Zhang, Guifeng]'s Articles
[Li, Suping]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.