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Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography
Alternative TitleBiotechnol. J.
Tan, Tianwei3; Su, Zhi-Guo2; Gu, Ming2; Xu, Jun3; Janson, Jan-Christer1
2010-05-01
Source PublicationBIOTECHNOLOGY JOURNAL
ISSN1860-6768
Volume5Issue:5Pages:505-510
AbstractFollowing its market introduction in 1982, the cross-linked 12% agarose gel media Superose 12 has become widely known as a tool for size exclusion chromatography of proteins and other biological macromolecules. In this review it is shown that, when appropriate mobile phases are used, Superose possesses adsorption properties similar to that of traditional media for hydrophilic interaction liquid chromatography (HILIC). This is illustrated by the separation and purification of low molecular weight compounds such as polyphenols including active components of traditional Chinese medicinal herbs and green tea. Structural features of the cross-linked agarose that likely cause the observed adsorption effects are discussed aswell. These are identified as being primarily ether bonds acting as strong hydrogen bond acceptors as well as hydrophobic residues originating from the cross-linking reagents.; Following its market introduction in 1982, the cross-linked 12% agarose gel media Superose 12 has become widely known as a tool for size exclusion chromatography of proteins and other biological macromolecules. In this review it is shown that, when appropriate mobile phases are used, Superose possesses adsorption properties similar to that of traditional media for hydrophilic interaction liquid chromatography (HILIC). This is illustrated by the separation and purification of low molecular weight compounds such as polyphenols including active components of traditional Chinese medicinal herbs and green tea. Structural features of the cross-linked agarose that likely cause the observed adsorption effects are discussed aswell. These are identified as being primarily ether bonds acting as strong hydrogen bond acceptors as well as hydrophobic residues originating from the cross-linking reagents.
KeywordCross-linked Agarose Green Tea Catechin Hilic Mixed-mode Separation Polyphenol Separation
SubtypeReview
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1002/biot.201000017
URL查看原文
Indexed BySCI
Language英语
WOS KeywordBOND ADSORPTION CHROMATOGRAPHY ; ONE-STEP PURIFICATION ; 12-PERCENT AGAROSE ; GEL-FILTRATION ; MEDIA ; PARTICLES ; GALLATE
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemical Research Methods ; Biotechnology & Applied Microbiology
WOS IDWOS:000278461800011
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Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6291
Collection研究所(批量导入)
Affiliation1.Uppsala Univ, Uppsala Biomed Ctr, Dept Phys & Analyt Chem, SE-75123 Uppsala, Sweden
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
3.Beijing Univ Chem Technol, Coll Life Sci & Technol, Dept Bioengn, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Tan, Tianwei,Su, Zhi-Guo,Gu, Ming,et al. Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography[J]. BIOTECHNOLOGY JOURNAL,2010,5(5):505-510.
APA Tan, Tianwei,Su, Zhi-Guo,Gu, Ming,Xu, Jun,&Janson, Jan-Christer.(2010).Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography.BIOTECHNOLOGY JOURNAL,5(5),505-510.
MLA Tan, Tianwei,et al."Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography".BIOTECHNOLOGY JOURNAL 5.5(2010):505-510.
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