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EFFECT OF VARIOUS FREEZING SOLUTIONS ON CRYOPRESERVATION OF MESENCHYMAL STEM CELLS FROM DIFFERENT ANIMAL SPECIES
Alternative TitleCryoLetters
Liu, Yang1,2,3; Xu, Xia1,4; Ma, Xuehu3; Liu, Jing2; Cui, Zhanfeng1
2011-09-01
Source PublicationCRYOLETTERS
ISSN0143-2044
Volume32Issue:5Pages:425-435
AbstractThe objective of this study is to compare the effects of different well defined freezing solutions with a reduced concentration of dimethylsulfoxide (DMSO) combined with polyethylene glycol (PEG) and/or trehalose on cryopreservation of mesenchymal stem cells (MSCs) from mice, rats and calves. Post-thaw cell viability, proliferation capacity and differentiation potential of MSCs from different species were assessed after cryopreservation with the conventional slow freezing method. Although the post-thaw viabilities and metabolic activities varied among the different species, satisfactory results were obtained with 5% (v/v) DMSO, 2% (w/v) PEG, 3% (w/v) trehalose and 2% (w/v) bovine serum albumin (BSA) as the freezing solution. Our results showed that mouse MSCs were more robust to cryopreservation compared with rat and bovine MSCs.; The objective of this study is to compare the effects of different well defined freezing solutions with a reduced concentration of dimethylsulfoxide (DMSO) combined with polyethylene glycol (PEG) and/or trehalose on cryopreservation of mesenchymal stem cells (MSCs) from mice, rats and calves. Post-thaw cell viability, proliferation capacity and differentiation potential of MSCs from different species were assessed after cryopreservation with the conventional slow freezing method. Although the post-thaw viabilities and metabolic activities varied among the different species, satisfactory results were obtained with 5% (v/v) DMSO, 2% (w/v) PEG, 3% (w/v) trehalose and 2% (w/v) bovine serum albumin (BSA) as the freezing solution. Our results showed that mouse MSCs were more robust to cryopreservation compared with rat and bovine MSCs.
KeywordCryopreservation Mesenchymal Stem Cells Well Defined Freezing Solutions
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordBONE-MARROW ; VIABILITY ; PERMEABILITY ; TREHALOSE ; RECOVERY ; RABBIT ; TISSUE ; SPERM ; SIZE
WOS Research AreaLife Sciences & Biomedicine - Other Topics ; Physiology
WOS SubjectBiology ; Physiology
WOS IDWOS:000296681200007
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6312
Collection研究所(批量导入)
Affiliation1.Univ Oxford, Dept Engn Sci, Inst Biomed Engn, Oxford OX1 3PJ, England
2.Dalian Med Univ, Affiliated Hosp 1, Sino UK Regenerat Med Ctr, Dalian 116011, Peoples R China
3.Dalian Univ Technol, Sch Chem Engn, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian 116024, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yang,Xu, Xia,Ma, Xuehu,et al. EFFECT OF VARIOUS FREEZING SOLUTIONS ON CRYOPRESERVATION OF MESENCHYMAL STEM CELLS FROM DIFFERENT ANIMAL SPECIES[J]. CRYOLETTERS,2011,32(5):425-435.
APA Liu, Yang,Xu, Xia,Ma, Xuehu,Liu, Jing,&Cui, Zhanfeng.(2011).EFFECT OF VARIOUS FREEZING SOLUTIONS ON CRYOPRESERVATION OF MESENCHYMAL STEM CELLS FROM DIFFERENT ANIMAL SPECIES.CRYOLETTERS,32(5),425-435.
MLA Liu, Yang,et al."EFFECT OF VARIOUS FREEZING SOLUTIONS ON CRYOPRESERVATION OF MESENCHYMAL STEM CELLS FROM DIFFERENT ANIMAL SPECIES".CRYOLETTERS 32.5(2011):425-435.
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