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Feasible plant regeneration in black pepper from petiole explants
Alternative TitleJ. Med. Plants Res.
Ahmad, Nisar1,4; Guo, Bin2; Fazal, Hina3,5; Abbasi, Bilal Haider1; Liu, Chun-Zhao6; Mahmood, Tariq3; Shinwari, Zabta Khan1
2011-09-16
Source PublicationJOURNAL OF MEDICINAL PLANTS RESEARCH
ISSN1996-0875
Volume5Issue:18Pages:4590-4595
AbstractA feasible plant regeneration protocol was established for economically important plant, black pepper. Callogenesis was induced from petiole explants of potted plants incubated on murashige and skoog (MS) - medium supplemented with different concentrations of several phytohormones (PGRs). The best callus induction (85%) was observed for MS-medium supplemented with 0.5 mg l(-1) 6 - benzyladenine (BA) after 04 weeks of culture. Subsequent transfer of callus to MS medium containing similar PGRs induced shoot regeneration. Highest shoot regeneration (92%) was recorded for 0.5 mg l(-1) BA after 5 weeks of transfer. Furthermore, 8.1 shoots/explant were recorded for 0.5 mg l(-1) BA. Addition of 2.0 mg l(-1) of indole-3-butyric acid (IBA) produced 5.1 cm long shoots with 85% shoot organogenesis. Shoots produced healthy plantlets when transferred to MS medium containing several concentrations of indole butyric acid IBA. Regenerated plantlets were transferred to pots for acclimatization.; A feasible plant regeneration protocol was established for economically important plant, black pepper. Callogenesis was induced from petiole explants of potted plants incubated on murashige and skoog (MS) - medium supplemented with different concentrations of several phytohormones (PGRs). The best callus induction (85%) was observed for MS-medium supplemented with 0.5 mg l(-1) 6 - benzyladenine (BA) after 04 weeks of culture. Subsequent transfer of callus to MS medium containing similar PGRs induced shoot regeneration. Highest shoot regeneration (92%) was recorded for 0.5 mg l(-1) BA after 5 weeks of transfer. Furthermore, 8.1 shoots/explant were recorded for 0.5 mg l(-1) BA. Addition of 2.0 mg l(-1) of indole-3-butyric acid (IBA) produced 5.1 cm long shoots with 85% shoot organogenesis. Shoots produced healthy plantlets when transferred to MS medium containing several concentrations of indole butyric acid IBA. Regenerated plantlets were transferred to pots for acclimatization.
KeywordPiper Nigrum Callus Organogenesis Petiole 6-benzyladenine
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPIPER-NIGRUM L. ; MEDICINAL-PLANT ; SOMATIC EMBRYOGENESIS ; SHOOT REGENERATION ; LEAF EXPLANTS ; ANTIOXIDANT ; CULTURES ; MICROPROPAGATION ; TISSUES ; SEEDS
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal
WOS IDWOS:000299778500031
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6346
Collection研究所(批量导入)
Affiliation1.Quaid I Azam Univ, Fac Biol Sci, Dept Biotechnol, Islamabad 45320, Pakistan
2.NW Univ Xian, Sch Life Sci, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Peoples R China
3.Quaid I Azam Univ, Fac Biol Sci, Dept Plant Sci, Islamabad 45320, Pakistan
4.Nucl Inst Food & Agr, Tarnab Peshawar 2500, Pakistan
5.Pakistan Council Sci & Ind Res PCSIR Labs Complex, Peshawar 2500, Pakistan
6.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Ahmad, Nisar,Guo, Bin,Fazal, Hina,et al. Feasible plant regeneration in black pepper from petiole explants[J]. JOURNAL OF MEDICINAL PLANTS RESEARCH,2011,5(18):4590-4595.
APA Ahmad, Nisar.,Guo, Bin.,Fazal, Hina.,Abbasi, Bilal Haider.,Liu, Chun-Zhao.,...&Shinwari, Zabta Khan.(2011).Feasible plant regeneration in black pepper from petiole explants.JOURNAL OF MEDICINAL PLANTS RESEARCH,5(18),4590-4595.
MLA Ahmad, Nisar,et al."Feasible plant regeneration in black pepper from petiole explants".JOURNAL OF MEDICINAL PLANTS RESEARCH 5.18(2011):4590-4595.
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