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Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification
Alternative TitleChin. J. Catal.
Jiang Yanjun1,2; Wang Qi1; Wang Wenqin1; Zhou Liya1; Gao Jing1
2012-05-01
Source PublicationCHINESE JOURNAL OF CATALYSIS
ISSN0253-9837
Volume33Issue:5Pages:857-862
AbstractThe hybrid biocatalyst was prepared by the combination of cross-linked enzyme aggregates (CLEAs) and biomimetic silicification, where the lipase Candida sp. 99-125 was used as model enzyme. The preparation conditions of lipase-CLEAs were optimized and the resulting activity of 771 U/g was obtained. Under the optimum conditions, 75% of the activity recovery was obtained. P/CLEAs was prepared by coprecipitation of lipase with polyethylene-imine (PEI), and the resulting activity was 897 U/g. The activity recovery of P/CLEAs was about 88%. The silica coating was formed on the outside surface of P/CLEAs via the induction of PEI. The resulting Coated-CLEAs showed high reusability. The stability of the Coated-CLEAs, including the resistance to trypsin and organic solvents, was also significantly improved.; The hybrid biocatalyst was prepared by the combination of cross-linked enzyme aggregates (CLEAs) and biomimetic silicification, where the lipase Candida sp. 99-125 was used as model enzyme. The preparation conditions of lipase-CLEAs were optimized and the resulting activity of 771 U/g was obtained. Under the optimum conditions, 75% of the activity recovery was obtained. P/CLEAs was prepared by coprecipitation of lipase with polyethylene-imine (PEI), and the resulting activity was 897 U/g. The activity recovery of P/CLEAs was about 88%. The silica coating was formed on the outside surface of P/CLEAs via the induction of PEI. The resulting Coated-CLEAs showed high reusability. The stability of the Coated-CLEAs, including the resistance to trypsin and organic solvents, was also significantly improved.
KeywordCross-linked Enzyme Aggregate Biomimetic Silicification Lipase Candida Sp 99-125 Genipin Immobilized Enzyme
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
DOI10.3724/SP.J.1088.2012.11055
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSTABILIZATION ; ACYLASE ; LINKING ; BIOCATALYST ; SUPPORTS ; SILICA
WOS Research AreaChemistry ; Engineering
WOS SubjectChemistry, Applied ; Chemistry, Physical ; Engineering, Chemical
WOS IDWOS:000304019200017
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6450
Collection研究所(批量导入)
Affiliation1.Hebei Univ Technol, Sch Chem Engn, Tianjin 300130, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Jiang Yanjun,Wang Qi,Wang Wenqin,et al. Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification[J]. CHINESE JOURNAL OF CATALYSIS,2012,33(5):857-862.
APA Jiang Yanjun,Wang Qi,Wang Wenqin,Zhou Liya,&Gao Jing.(2012).Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification.CHINESE JOURNAL OF CATALYSIS,33(5),857-862.
MLA Jiang Yanjun,et al."Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification".CHINESE JOURNAL OF CATALYSIS 33.5(2012):857-862.
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