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The Production of Hydroxyl Radical in HRP-NADH-H2O2/O-2 Systems and Its Application in Chlorobenzene Removal
Alternative TitleSpectrosc. Spectr. Anal.
Li Hai Tao1,2; Li Yu-ping1; Cao Hong Bin1; Li Xin Gang2; Xue Zhan Qiang3
2010-11-01
Source PublicationSPECTROSCOPY AND SPECTRAL ANALYSIS
ISSN1000-0593
Volume30Issue:11Pages:3119-3123
AbstractThe mechanism of radical generation in HRP NADHO(2)/H2O2 systems and state-change of horseradish peroxidase (HRP) was investigated by using ESR and UV measurements and the novel enzyme-coenzymatic systems were performed to de grade chlorobenzene as a non phenolic persistent organic pollutants The UV results showed that compound III was produced from HRP oxidized by hydrogen peroxide with the catalysis of NADH which would generate hydroxyl radical The ESR results demonstrated the production of OH and O-2(-) in enzyme-coenzymatic system in the presence of O-2 or H2O2 with DMPO and POBN as spin-trappers respectively In HRP NADH-H2O2 system compound III was the main state of HRP in the initial 10 min and then converted to HRP with generating hydroxyl radical and after the addition of oxygen the production of hydroxyl radical was promoted rapidly as 4 times as that of the system in absence of oxygen. The addition of SOD(Zn Cu) decreased the production of hydroxyl radical significantly resulting from that SOD eli minated O-2 reduction to O-2(-) by NADH and then inhibited OH formation. The results showed that NADH could improve by about 20% enzyme activity of HRP for phenol removal The removal of chlorobenzene with HRP NADH H2O2 and HRP NADH H2O2 O-2 systems reached 24 6% and 48 2% respectively which was much higher than that with traditional enzymatic system (1 42%) showing a promising prospect in proposal of other non phenol POPs in wastewater; The mechanism of radical generation in HRP NADHO(2)/H2O2 systems and state-change of horseradish peroxidase (HRP) was investigated by using ESR and UV measurements and the novel enzyme-coenzymatic systems were performed to de grade chlorobenzene as a non phenolic persistent organic pollutants The UV results showed that compound III was produced from HRP oxidized by hydrogen peroxide with the catalysis of NADH which would generate hydroxyl radical The ESR results demonstrated the production of OH and O-2(-) in enzyme-coenzymatic system in the presence of O-2 or H2O2 with DMPO and POBN as spin-trappers respectively In HRP NADH-H2O2 system compound III was the main state of HRP in the initial 10 min and then converted to HRP with generating hydroxyl radical and after the addition of oxygen the production of hydroxyl radical was promoted rapidly as 4 times as that of the system in absence of oxygen. The addition of SOD(Zn Cu) decreased the production of hydroxyl radical significantly resulting from that SOD eli minated O-2 reduction to O-2(-) by NADH and then inhibited OH formation. The results showed that NADH could improve by about 20% enzyme activity of HRP for phenol removal The removal of chlorobenzene with HRP NADH H2O2 and HRP NADH H2O2 O-2 systems reached 24 6% and 48 2% respectively which was much higher than that with traditional enzymatic system (1 42%) showing a promising prospect in proposal of other non phenol POPs in wastewater
KeywordHydroxyl Radical Horseradish Peroxidase Coenzyme Epr Wastewater Treatment
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.3964/j.issn.1000.0593(2010)11.3119.05
URL查看原文
Indexed BySCI
Language英语
WOS KeywordHORSERADISH-PEROXIDASE ; HYDROGEN-PEROXIDE ; PENTACHLOROPHENOL ; OXIDATION ; PHENOLS
WOS Research AreaSpectroscopy
WOS SubjectSpectroscopy
WOS IDWOS:000285284000053
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6458
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
2.Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China
3.Angang Steel Co Ltd, Chem Plant, Anshan 114021, Peoples R China
Recommended Citation
GB/T 7714
Li Hai Tao,Li Yu-ping,Cao Hong Bin,et al. The Production of Hydroxyl Radical in HRP-NADH-H2O2/O-2 Systems and Its Application in Chlorobenzene Removal[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2010,30(11):3119-3123.
APA Li Hai Tao,Li Yu-ping,Cao Hong Bin,Li Xin Gang,&Xue Zhan Qiang.(2010).The Production of Hydroxyl Radical in HRP-NADH-H2O2/O-2 Systems and Its Application in Chlorobenzene Removal.SPECTROSCOPY AND SPECTRAL ANALYSIS,30(11),3119-3123.
MLA Li Hai Tao,et al."The Production of Hydroxyl Radical in HRP-NADH-H2O2/O-2 Systems and Its Application in Chlorobenzene Removal".SPECTROSCOPY AND SPECTRAL ANALYSIS 30.11(2010):3119-3123.
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