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Rapid concentration of protein solution by a crossflow electro-ultrafiltration process
Alternative TitleSep. Purif. Technol.
Song, Weijie1,2; Su, Yi1; Chen, Xiangrong1; Ding, Luhui3; Wan, Yinhua1
2010-06-18
Source PublicationSEPARATION AND PURIFICATION TECHNOLOGY
ISSN1383-5866
Volume73Issue:2Pages:310-318
AbstractAn electro-ultrafiltration process was proposed and developed for concentrating protein solution rapidly by applying an electric field and employing a membrane with a higher nominal molecular weight cut-off (NMWCO) than the molecular weight of protein to be concentrated. In this process, the high NMWCO membrane was used to achieve a high permeate flux while the adoption of an electric field was used to obtain a high rejection of the target protein by manipulating the electrophoretic effect. Bovine serum albumen (BSA) was used as a model protein, and 50 and 100 kDa NMWCO polysulphone (PS) membranes were used for BSA concentration. The effects of membrane NMWCO, electric field strength, feed concentration on permeate flux and protein rejection were examined. The experimental results showed that the concentration time required for a target protein concentration could be significantly shortened when an electric field was applied and a membrane with higher NMWCO was used. When a 3000 V m(-1) electric field was applied and the 100 kDa NMWCO polysulphone (PS) membrane was used, the concentration time for concentrating 0.5 g L(-1) BSA solution to around 1.0 g L(-1) decreased by 80% as compared with the process with a 50 kDa membrane without electric field, suggesting that this electro-ultrafiltration concentration process is a viable alternative to conventional ultrafiltration process and a promising method for concentrating dilute protein solutions. (C) 2010 Elsevier B.V. All rights reserved.; An electro-ultrafiltration process was proposed and developed for concentrating protein solution rapidly by applying an electric field and employing a membrane with a higher nominal molecular weight cut-off (NMWCO) than the molecular weight of protein to be concentrated. In this process, the high NMWCO membrane was used to achieve a high permeate flux while the adoption of an electric field was used to obtain a high rejection of the target protein by manipulating the electrophoretic effect. Bovine serum albumen (BSA) was used as a model protein, and 50 and 100 kDa NMWCO polysulphone (PS) membranes were used for BSA concentration. The effects of membrane NMWCO, electric field strength, feed concentration on permeate flux and protein rejection were examined. The experimental results showed that the concentration time required for a target protein concentration could be significantly shortened when an electric field was applied and a membrane with higher NMWCO was used. When a 3000 V m(-1) electric field was applied and the 100 kDa NMWCO polysulphone (PS) membrane was used, the concentration time for concentrating 0.5 g L(-1) BSA solution to around 1.0 g L(-1) decreased by 80% as compared with the process with a 50 kDa membrane without electric field, suggesting that this electro-ultrafiltration concentration process is a viable alternative to conventional ultrafiltration process and a promising method for concentrating dilute protein solutions. (C) 2010 Elsevier B.V. All rights reserved.
KeywordElectra-ultrafiltration Concentration Polarization Fouling Concentration
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPULSED-ELECTRIC-FIELD ; BOVINE SERUM-ALBUMIN ; COLLOIDAL SOLUTION ; ULTRAFILTRATION ; MEMBRANE
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
WOS IDWOS:000279236300030
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6467
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Univ Technol Compiegne, Biol Engn Dept, EA 4297TIMR, F-60205 Compiegne, France
Recommended Citation
GB/T 7714
Song, Weijie,Su, Yi,Chen, Xiangrong,et al. Rapid concentration of protein solution by a crossflow electro-ultrafiltration process[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2010,73(2):310-318.
APA Song, Weijie,Su, Yi,Chen, Xiangrong,Ding, Luhui,&Wan, Yinhua.(2010).Rapid concentration of protein solution by a crossflow electro-ultrafiltration process.SEPARATION AND PURIFICATION TECHNOLOGY,73(2),310-318.
MLA Song, Weijie,et al."Rapid concentration of protein solution by a crossflow electro-ultrafiltration process".SEPARATION AND PURIFICATION TECHNOLOGY 73.2(2010):310-318.
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