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A Simple and Rapid Procedure for Purification of Haptoglobin from Human Plasma Fraction IV
Alternative TitleArtif. Cells Blood Substit. Biotechnol.
Sun, Lijing1,2; Huang, Yongdong1; Zhang, Yan1,2; Meng, Qingqiang1,2; Luo, Jian1; Fan, Bei3; Ma, Guanghui1; Su, Zhiguo1
2011-04-01
Source PublicationARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
ISSN1073-1199
Volume39Issue:2Pages:79-86
AbstractHuman plasma fraction IV is an intermediate precipitate during the production of human serum albumin using cold ethanol method. Haptoglobin locates in this fraction can be purified for various applications. A new process integration of polyethylene glycol (PEG) precipitation and ion-exchange chromatography (IEC) was developed for purification of haptoglobin, which could effectively purify the haptoglobin from 16.6% to 95%. The recovery of the new process was 58.2% in comparison to 30.3% of the conventional affinity chromatography. Furthermore, 175 mg haptoglobin production in a scaled-up process showed the method to be simple, fast, and low-cost.; Human plasma fraction IV is an intermediate precipitate during the production of human serum albumin using cold ethanol method. Haptoglobin locates in this fraction can be purified for various applications. A new process integration of polyethylene glycol (PEG) precipitation and ion-exchange chromatography (IEC) was developed for purification of haptoglobin, which could effectively purify the haptoglobin from 16.6% to 95%. The recovery of the new process was 58.2% in comparison to 30.3% of the conventional affinity chromatography. Furthermore, 175 mg haptoglobin production in a scaled-up process showed the method to be simple, fast, and low-cost.
KeywordHaptoglobin Human Plasma Fraction Iv Purification Peg Precipitation Ion-exchange Chromatography
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.3109/10731199.2010.509705
URL查看原文
Indexed BySCI
Language英语
WOS KeywordANTIBODY AFFINITY-CHROMATOGRAPHY ; ION-EXCHANGE CHROMATOGRAPHY ; BORDETELLA-PERTUSSIS ; AMMONIUM-SULFATE ; PRECIPITATION ; HEMOGLOBIN ; SERUM ; POLYMORPHISM ; TOXIN
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering ; Materials Science
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials
WOS IDWOS:000288022100004
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6480
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100190, Peoples R China
3.Hualan Biol Engn Inc, Xinxiang, Peoples R China
Recommended Citation
GB/T 7714
Sun, Lijing,Huang, Yongdong,Zhang, Yan,et al. A Simple and Rapid Procedure for Purification of Haptoglobin from Human Plasma Fraction IV[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,2011,39(2):79-86.
APA Sun, Lijing.,Huang, Yongdong.,Zhang, Yan.,Meng, Qingqiang.,Luo, Jian.,...&Su, Zhiguo.(2011).A Simple and Rapid Procedure for Purification of Haptoglobin from Human Plasma Fraction IV.ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,39(2),79-86.
MLA Sun, Lijing,et al."A Simple and Rapid Procedure for Purification of Haptoglobin from Human Plasma Fraction IV".ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY 39.2(2011):79-86.
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