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PEGylation of rhIL-1RA increased its solution stability at room temperature
Yu, Pengzhan1,2; Qin, Dan2; Qin, Guohong1,2; Fan, Bei1; Ma, Guanghui1; Su, Zhiguo1; Su, ZG
2009-12-01
Source PublicationPROCESS BIOCHEMISTRY
ISSN1359-5113
Volume44Issue:12Pages:1340-1345
AbstractRecombinant human interleukin-1 receptor antagonist (rhIL-1RA) is an important cytokine in the treatment of inflammatory diseases. However, it is instable in aqueous solution and prone to degrade without the addition of any excipient. Following the 30- or 60-day storage in 50 mM sodium acetate (pH 5.0) at room temperature, rhIL-1RA markedly degraded into three species (denoted as P1, P2 and P3 in this study), the bioactivities of which to a different extent was lost (from 9.72 x 104 Ul/mg to 3.07 x 10(3) Ul/mg for P1, 5.49 x 10(3) Ul/mg for P2, 1.09 X 10(4) Ul/mg for P3, respectively). To solve this problem, we prepared the mono-PEGylated rhIL-IRA with propionaldehyde mPEG (ALD-PEG, M(w) 5000 Da). The conjugate showed more favorable stability than original protein, and remained homogeneous under the similar storage conditions. In addition, the activity of the conjugate was well retained (from 5.80 x 10(4) Ul/mg to 4.92 X 10(4) Ul/mg), compared to that of original protein. The results based on the combination analysis of CD, ion exchange chromatography and RP-HPLC, revealed that the stability improvement of rhIL-1RA majorly benefited from the PEG strands protection against the protein conformational changes occurred during the storage. (C) 2009 Published by Elsevier Ltd.
KeywordRecombinant Human Interleukin-1 Receptor Antagonist Pegylation Aqueous Stability Conformational Changes Chromatography
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.procbio.2009.07.010
Indexed BySCI
Language英语
WOS KeywordINTERLEUKIN-1 RECEPTOR ANTAGONIST ; AGGREGATION ; FORMULATION ; IL-1 ; THERMOSTABILITY ; KINETICS ; BINDING ; GROWTH ; ASSAY
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000272071900006
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6539
Collection生化工程国家重点实验室
Corresponding AuthorSu, ZG
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Jiangsu Simcere Pharmaceut R&D Co Ltd, Beijing 100007, Peoples R China
Recommended Citation
GB/T 7714
Yu, Pengzhan,Qin, Dan,Qin, Guohong,et al. PEGylation of rhIL-1RA increased its solution stability at room temperature[J]. PROCESS BIOCHEMISTRY,2009,44(12):1340-1345.
APA Yu, Pengzhan.,Qin, Dan.,Qin, Guohong.,Fan, Bei.,Ma, Guanghui.,...&Su, ZG.(2009).PEGylation of rhIL-1RA increased its solution stability at room temperature.PROCESS BIOCHEMISTRY,44(12),1340-1345.
MLA Yu, Pengzhan,et al."PEGylation of rhIL-1RA increased its solution stability at room temperature".PROCESS BIOCHEMISTRY 44.12(2009):1340-1345.
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