| A novel stationary phase derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed protein chromatography | |
| Qu, Jian-Bo1,2; Wan, Xing-Zhong3; Zhai, Yan-Qin1,2; Zhou, Wei-Qing1; Su, Zhi-Guo1; Ma, Guang-Hui1; Ma, GH | |
| 2009-09-11 | |
| Source Publication | JOURNAL OF CHROMATOGRAPHY A
 |
| ISSN | 0021-9673 |
| Volume | 1216Issue:37Pages:6511-6516 |
| Abstract | Using agarose coated gigaporous polystyrene microspheres as a base support, a novel anion exchanger (DEAE-AP) has been developed after functionalization with diethylaminoethyl chloride. The gigaporous structure, static adsorption behavior, and chromatographic properties of DEAE-AP medium were characterized and compared with those of commercially available resin DEAE Sepharose Fast Flow (DEAE-FF). The results implied that there existed some through pores in DEAE-AP microspheres, which effectively reduced resistance to stagnant mobile phase mass transfer by inducing convective flow of mobile phase in the gigapores of medium. As a consequence, the column packed with DEAE-AP exhibited low column backpressure, high column efficiency, high dynamic binding capacity and high protein resolution at high flow velocity up to 2600cm/h. In conclusion, all the results suggested that the gigaporous absorbent is promising for high-speed protein chromatography. (C) 2009 Elsevier B.V. All rights reserved. |
| Keyword | Gigaporous Polystyrene Microspheres Anion Exchanger Protein Chromatography Column Efficiency Dynamic Binding Capacity |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1016/j.chroma.2009.07.059 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | PERFUSION CHROMATOGRAPHY ; SEPARATION ; PURIFICATION ; SUPPORTS |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemical Research Methods ; Chemistry, Analytical |
| WOS ID | WOS:000269631500007 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/6583 |
| Collection | 生化工程国家重点实验室 |
| Corresponding Author | Ma, GH |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Beijing Baiao Pharmaceut Co Ltd, Beijing 102200, Peoples R China |
| Recommended Citation GB/T 7714 | Qu, Jian-Bo,Wan, Xing-Zhong,Zhai, Yan-Qin,et al. A novel stationary phase derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed protein chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(37):6511-6516. |
| APA | Qu, Jian-Bo.,Wan, Xing-Zhong.,Zhai, Yan-Qin.,Zhou, Wei-Qing.,Su, Zhi-Guo.,...&Ma, GH.(2009).A novel stationary phase derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed protein chromatography.JOURNAL OF CHROMATOGRAPHY A,1216(37),6511-6516. |
| MLA | Qu, Jian-Bo,et al."A novel stationary phase derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed protein chromatography".JOURNAL OF CHROMATOGRAPHY A 1216.37(2009):6511-6516. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 20131129-62-复件 1-s2.（935KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment