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A novel stationary phase derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed protein chromatography
Qu, Jian-Bo1,2; Wan, Xing-Zhong3; Zhai, Yan-Qin1,2; Zhou, Wei-Qing1; Su, Zhi-Guo1; Ma, Guang-Hui1; Ma, GH
2009-09-11
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1216Issue:37Pages:6511-6516
AbstractUsing agarose coated gigaporous polystyrene microspheres as a base support, a novel anion exchanger (DEAE-AP) has been developed after functionalization with diethylaminoethyl chloride. The gigaporous structure, static adsorption behavior, and chromatographic properties of DEAE-AP medium were characterized and compared with those of commercially available resin DEAE Sepharose Fast Flow (DEAE-FF). The results implied that there existed some through pores in DEAE-AP microspheres, which effectively reduced resistance to stagnant mobile phase mass transfer by inducing convective flow of mobile phase in the gigapores of medium. As a consequence, the column packed with DEAE-AP exhibited low column backpressure, high column efficiency, high dynamic binding capacity and high protein resolution at high flow velocity up to 2600cm/h. In conclusion, all the results suggested that the gigaporous absorbent is promising for high-speed protein chromatography. (C) 2009 Elsevier B.V. All rights reserved.
KeywordGigaporous Polystyrene Microspheres Anion Exchanger Protein Chromatography Column Efficiency Dynamic Binding Capacity
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2009.07.059
Indexed BySCI
Language英语
WOS KeywordPERFUSION CHROMATOGRAPHY ; SEPARATION ; PURIFICATION ; SUPPORTS
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000269631500007
Citation statistics
Cited Times:40[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6583
Collection生化工程国家重点实验室
Corresponding AuthorMa, GH
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Beijing Baiao Pharmaceut Co Ltd, Beijing 102200, Peoples R China
Recommended Citation
GB/T 7714
Qu, Jian-Bo,Wan, Xing-Zhong,Zhai, Yan-Qin,et al. A novel stationary phase derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed protein chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(37):6511-6516.
APA Qu, Jian-Bo.,Wan, Xing-Zhong.,Zhai, Yan-Qin.,Zhou, Wei-Qing.,Su, Zhi-Guo.,...&Ma, GH.(2009).A novel stationary phase derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed protein chromatography.JOURNAL OF CHROMATOGRAPHY A,1216(37),6511-6516.
MLA Qu, Jian-Bo,et al."A novel stationary phase derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed protein chromatography".JOURNAL OF CHROMATOGRAPHY A 1216.37(2009):6511-6516.
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