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Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066. Part I: Process development
Tang, Ya-Jie1,2,3; Li, Yan2; Zhong, Jian-Jiang4; Tang, YJ
2009-08-01
Source PublicationBIOPROCESS AND BIOSYSTEMS ENGINEERING
ISSN1615-7591
Volume32Issue:5Pages:663-671
AbstractA novel biotransformation process of podophyllotoxin (1) to produce picropodophyllotoxin (2) and podophyllic acid (3) was developed in this work. Eight bacteria which could modify the structure of podophyllotoxin were screened out from the tested fourteen bacteria. The highest conversion of podophyllotoxin (i.e., 70.2 +/- A 8.0%) was obtained when Pseudomonas aeruginosa CCTCC AB93066 was used as biocatalyst, so P. aeruginosa was selected as a typical biocatalyst in the following study. Product (2) and (3) were separated through D312 macroporous resin and sephadex LH-20 gel column chromatograph. On the basis of (1)H NMR, (13)C NMR, ESI-MS and Elemental Analysis, product (2) and (3) were identified as picropodophyllotoxin (2) and podophyllic acid (3), respectively. This suggested the site-specific isomerization and hydrolization of podophyllotoxin occurred during its biotransformation process by P. aeruginosa. For the first time, podophyllotoxin was biotransformed into its hydrolytic derivate (i.e., podophyllic acid).
KeywordBiotransformation Process Development Podophyllotoxin Podophyllic Acid Picropodophyllotoxin Pseudomonas Aeruginosa
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1007/s00449-008-0290-9
Indexed BySCI
Language英语
WOS KeywordSPIN-LABELED DERIVATIVES ; CELL-SUSPENSION CULTURES ; CONGENERS ; LIGNANS ; DEOXYPODOPHYLLOTOXIN ; BIOCONVERSION ; DISCOVERY ; ETOPOSIDE ; ANTITUMOR ; ANALOGS
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000268294600013
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6608
Collection生化工程国家重点实验室
Corresponding AuthorTang, YJ
Affiliation1.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
2.Hubei Univ Technol, Coll Bioengn, Key Lab Fermentat Engn, Minist Educ,Hubei Prov Key Lab Ind Microbiol, Wuhan 430068, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
4.Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Key Lab Microbial Metab, Minist Educ, Shanghai 200240, Peoples R China
Recommended Citation
GB/T 7714
Tang, Ya-Jie,Li, Yan,Zhong, Jian-Jiang,et al. Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066. Part I: Process development[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2009,32(5):663-671.
APA Tang, Ya-Jie,Li, Yan,Zhong, Jian-Jiang,&Tang, YJ.(2009).Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066. Part I: Process development.BIOPROCESS AND BIOSYSTEMS ENGINEERING,32(5),663-671.
MLA Tang, Ya-Jie,et al."Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066. Part I: Process development".BIOPROCESS AND BIOSYSTEMS ENGINEERING 32.5(2009):663-671.
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