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Study on the pH-Sensitive Secondary Structure of gamma-PGA Embedded with Magnetite Nanoparticles
Zhang Ju-hua1,2; Xing Jian-min1; Jiang Yang-yang1,2; Gao Hong-shuai1,2; Liu Hui-zhou1; Xing, JM
2009-08-01
Source PublicationSPECTROSCOPY AND SPECTRAL ANALYSIS
ISSN1000-0593
Volume29Issue:8Pages:2148-2151
AbstractThe conformational transition of poly gamma-glutamic acid (gamma-PGA) embedded with magnetite nanoparticles under various pH conditions was investigated by Fourier transform infrared spectroscopy (FTIR). The secondary structure content was determined through the analysis of amide I bands of Fourier deconvolution spectra, secondary derivative spectra and the Gaussian curve fitting of the original infrared spectra. The results showed that the conformation of the gamma-PGA was affected by solution pH. The total contents of beta-sheet and beta-turn were higher than 65%, while alpha-helix and random coil were low. The content of beta-turn increased with increasing pH, while the beta-sheet decreased. Additionally, the zeta potential results showed that the pH-sensitive secondary structure gamma-PGA had influence on the stability of suspension of magnetic gamma-PGA nanospheres. The minimum value of zeta potential (-35. 4 mV) was obtained at pH 10. 2.
KeywordFtir Poly Gamma-glutamic Acid Magnetic Nanospheres Ph Zeta Potential
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.3964/j.issn.1000-0593(2009)08-2148-04
Indexed BySCI
Language英语
WOS KeywordALPHA-AMINO-ACIDS ; L-GLUTAMIC ACID ; AQUEOUS-SOLUTIONS ; INFRARED-SPECTROSCOPY ; BETA-TURNS ; PEPTIDES ; POLYPEPTIDES ; PROTEINS ; CONFORMATION
WOS Research AreaSpectroscopy
WOS SubjectSpectroscopy
WOS IDWOS:000269998800031
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6628
Collection湿法冶金清洁生产技术国家工程实验室
Corresponding AuthorXing, JM
Affiliation1.Chinese Acad Sci, Key Lab Green Proc & Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang Ju-hua,Xing Jian-min,Jiang Yang-yang,et al. Study on the pH-Sensitive Secondary Structure of gamma-PGA Embedded with Magnetite Nanoparticles[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2009,29(8):2148-2151.
APA Zhang Ju-hua,Xing Jian-min,Jiang Yang-yang,Gao Hong-shuai,Liu Hui-zhou,&Xing, JM.(2009).Study on the pH-Sensitive Secondary Structure of gamma-PGA Embedded with Magnetite Nanoparticles.SPECTROSCOPY AND SPECTRAL ANALYSIS,29(8),2148-2151.
MLA Zhang Ju-hua,et al."Study on the pH-Sensitive Secondary Structure of gamma-PGA Embedded with Magnetite Nanoparticles".SPECTROSCOPY AND SPECTRAL ANALYSIS 29.8(2009):2148-2151.
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