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Effects of dissolved oxygen tension and agitation rate on the production of heat-shock protein glycoprotein 96 by MethA tumor cell suspension culture in stirred-tank bioreactors
Tang, Ya-Jie1,2,3; Li, Hong-Mei1,2; Hamel, Jean-Francois P.1; Tang, YJ
2009-06-01
Source PublicationBIOPROCESS AND BIOSYSTEMS ENGINEERING
ISSN1615-7591
Volume32Issue:4Pages:475-484
AbstractHeat-shock protein glycoprotein (gp96) serves as a natural adjuvant for chaperoning antigenic peptide into the immune surveillance pathway. In our laboratory, MethA tumor cell suspension culture process has been recently developed for gp96 production in spinner flask. In this work, effects of dissolved oxygen tension (DOT) and agitation rate on this process were studied in stirred-tank bioreactor. The optimal conditions for gp96 production were different with those for MethA tumor cell growth. MethA tumor cell growth pattern was not much changed by various levels of DOT and agitation rate, while gp96 biosynthesis was more sensitive to DOT and agitation rate. Compared with 50% of DOT, the production and specific productivity of gp96 was increased by 27 and 66% at 10% of DOT, respectively. Compared with the agitation rate of 100 rpm, the production and volumetric productivity of gp96 was increased by 48 and 144% at the agitation rate of 200 rpm, respectively. Low DOT (i.e., 10% of air saturation) and high agitation rate (i.e., 200 rpm) were identified to be favorable for gp96 biosynthesis. The results of this work might be useful to scale-up the bioprocess into the pilot scale.
KeywordHeat-shock Protein glycoProtein 96 Metha Tumor Cell Suspension Culture Stirred-tank Bioreactor Process Development Dissolved Oxygen Tension Agitation Rate
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1007/s00449-008-0267-8
Indexed BySCI
Language英语
WOS KeywordMONOCLONAL-ANTIBODY PRODUCTION ; STRESS-INDUCED PROTEINS ; GP96 PREPARATIONS ; RECOMBINANT PROTEIN ; PRODUCTION KINETICS ; PERFUSION CULTURE ; OXIDATIVE STRESS ; HYBRIDOMA GROWTH ; ANIMAL-CELLS ; CHO-CELLS
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000266238900006
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6652
Collection生化工程国家重点实验室
Corresponding AuthorTang, YJ
Affiliation1.MIT, Dept Chem Engn, Cambridge, MA 02139 USA
2.Hubei Univ Technol, Coll Bioengn, Hubei Prov Key Lab Ind Microbiol, Wuhan 430068, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Tang, Ya-Jie,Li, Hong-Mei,Hamel, Jean-Francois P.,et al. Effects of dissolved oxygen tension and agitation rate on the production of heat-shock protein glycoprotein 96 by MethA tumor cell suspension culture in stirred-tank bioreactors[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2009,32(4):475-484.
APA Tang, Ya-Jie,Li, Hong-Mei,Hamel, Jean-Francois P.,&Tang, YJ.(2009).Effects of dissolved oxygen tension and agitation rate on the production of heat-shock protein glycoprotein 96 by MethA tumor cell suspension culture in stirred-tank bioreactors.BIOPROCESS AND BIOSYSTEMS ENGINEERING,32(4),475-484.
MLA Tang, Ya-Jie,et al."Effects of dissolved oxygen tension and agitation rate on the production of heat-shock protein glycoprotein 96 by MethA tumor cell suspension culture in stirred-tank bioreactors".BIOPROCESS AND BIOSYSTEMS ENGINEERING 32.4(2009):475-484.
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