CAS OpenIR  > 生化工程国家重点实验室
Coupling recovery strategy of cellulase in hydrolyzate after hydrolysis with Tannin flocculation and PEG desorption
Xu, J.; Chen, H.; Xu, J
2009-05-01
Source PublicationAPPLIED BIOCHEMISTRY AND MICROBIOLOGY
ISSN0003-6838
Volume45Issue:3Pages:309-312
AbstractThrough determination of cellulase in terms of protein content in the hydrolyzate after hydrolysis, it was found that 50.15-67.21% of the originally added protein was still remained in the hydrolyzate. Tannin flocculation followed by PEG desorption was coupled to recover this portion of cellulase. About 94.99% of the protein in the hydrolyzate was precipitated into Tannin-cellulase compound under tannin concentration of 5% (w/v) with pH 5.0 and NaCl concentration of 1.0%. In order to desorb cellulase from Tannin-cellulase compound, PEG 6000 was employed as a desorbent. The recovered enzyme activity reached 133.25% when the PEG concentration was 1.5% (w/v). This, to some extent, indicated that PEG was also an effective activator of cellulase. The article is published in the original.
KeywordLignocellulosic Biomass Enzymatic-hydrolysis Newspaper
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1134/S0003683809030120
Indexed BySCI
Language英语
WOS KeywordLIGNOCELLULOSIC BIOMASS ; ENZYMATIC-HYDROLYSIS ; NEWSPAPER
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000265939300012
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6674
Collection生化工程国家重点实验室
Corresponding AuthorXu, J
AffiliationCAS, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Xu, J.,Chen, H.,Xu, J. Coupling recovery strategy of cellulase in hydrolyzate after hydrolysis with Tannin flocculation and PEG desorption[J]. APPLIED BIOCHEMISTRY AND MICROBIOLOGY,2009,45(3):309-312.
APA Xu, J.,Chen, H.,&Xu, J.(2009).Coupling recovery strategy of cellulase in hydrolyzate after hydrolysis with Tannin flocculation and PEG desorption.APPLIED BIOCHEMISTRY AND MICROBIOLOGY,45(3),309-312.
MLA Xu, J.,et al."Coupling recovery strategy of cellulase in hydrolyzate after hydrolysis with Tannin flocculation and PEG desorption".APPLIED BIOCHEMISTRY AND MICROBIOLOGY 45.3(2009):309-312.
Files in This Item:
File Name/Size DocType Version Access License
20131209-155-复件 S000(151KB) 限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xu, J.]'s Articles
[Chen, H.]'s Articles
[Xu, J]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xu, J.]'s Articles
[Chen, H.]'s Articles
[Xu, J]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xu, J.]'s Articles
[Chen, H.]'s Articles
[Xu, J]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.