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Systematic purification of polydatin, resveratrol and anthraglycoside B from Polygonum cuspidatum Sieb. et Zucc.
Zhang, Dalei1,2,3; Li, Xiunan1; Hao, Dongxia1; Li, Guisheng4; Xu, Benming4; Ma, Guanghui1; Su, Zhiguo1; Su, ZG
2009-04-20
Source PublicationSEPARATION AND PURIFICATION TECHNOLOGY
ISSN1383-5866
Volume66Issue:2Pages:329-339
AbstractFor comprehensive utilization of Polygonum cuspidatum Sieb. et Zucc., a systematic, environmental friendly preparative process was developed for purification of polydatin, resveratrol and anthraglycoside B simultaneously from the herb. The process was simple, consisting of macroporous resin adsorption and reversed-phase liquid chromatography. Macroporous resin adsorption separated the solvent extract into fraction I containing polydatin and fraction II containing resveratrol as well as anthraglycoside B. The fractions I and II were further purified by reversed-phase chromatography to get the fractions corresponding to polydatin, resveratrol and anthraglycoside B, respectively. Two polydivinylbenzene microspheres (PDVB-SPG and PDVB-Swelling) were prepared by SPG membrane emulsification and by two-step activated swelling polymerization, respectively. These new media were compared with a commercial C(18) bonded silica medium in the reversed-phase liquid chromatography step. The PDVB-SPG column demonstrated a better separation compared with C(18) and PDVB-Swelling column. The pH value of the mobile phase showed little effect on the separation of polydatin and resveratrol, and large effect on the separation of anthraglycoside B for both HPLC analysis and preparative reversed-phase liquid chromatography. After the chromatographic process and a further crystallization, polydatin, resveratrol and anthraglycoside B were purified from 7.5%, 1.3% and 2.4% to 98.8%, 98.2% and 98.6%, respectively, with total recoveries of 81.3%,77.4% and 80.2%. (C) 2009 Elsevier B.V. All rights reserved.
KeywordPolygonum Cuspidatum Sieb. Et Zucc. Polyclatin Resveratrol Anthraglycoside b Purification
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1016/j.seppur.2008.12.013
Indexed BySCI
Language英语
WOS KeywordCOUNTER-CURRENT CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; SEPARATION ; COPOLYMERS ; RETENTION ; PICEID ; ACIDS
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
WOS IDWOS:000265515700017
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6682
Collection生化工程国家重点实验室
Corresponding AuthorSu, ZG
Affiliation1.Chinese Acad Sci, Natl Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Shandong Engn Res Ctr Nat Drugs, Yantai 264003, Shandong, Peoples R China
4.Yantai Univ, Sch Pharm, Yantai 264005, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Dalei,Li, Xiunan,Hao, Dongxia,et al. Systematic purification of polydatin, resveratrol and anthraglycoside B from Polygonum cuspidatum Sieb. et Zucc.[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2009,66(2):329-339.
APA Zhang, Dalei.,Li, Xiunan.,Hao, Dongxia.,Li, Guisheng.,Xu, Benming.,...&Su, ZG.(2009).Systematic purification of polydatin, resveratrol and anthraglycoside B from Polygonum cuspidatum Sieb. et Zucc..SEPARATION AND PURIFICATION TECHNOLOGY,66(2),329-339.
MLA Zhang, Dalei,et al."Systematic purification of polydatin, resveratrol and anthraglycoside B from Polygonum cuspidatum Sieb. et Zucc.".SEPARATION AND PURIFICATION TECHNOLOGY 66.2(2009):329-339.
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