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Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066, Part II: Process optimization
Li, Yan1; Li, Yuan-Yuan1; Mi, Zhi-Yuan1; Li, Dong-Sheng1; Tang, Ya-Jie1,2,3; Tang, YJ
2009-04-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume100Issue:7Pages:2271-2277
AbstractThis work optimized the novel biotransformation process of podophyllotoxin to produce podophyllic acid by Pseudomonas aeruginosa CCTCC AB93066. Firstly, the biotransformation process was significantly affected by medium composition. 5 g/l of yeast extract and 5 g/l of peptone were favorable for podophyllic acid production (i.e. 25.3 +/- 3.7 mg/l), while not beneficial for the cell growth of P. aeruginosa. This indicated that the accumulation of podophyllic acid was not corresponded well to the cell growth of P. aeruginosa. 0 g/l of sucrose was beneficial for podophyllic acid production (i.e. 34.3 +/- 3.9 mg/l), which led to high podophyllotoxin conversion (i.e. 98.2 +/- 0.1%). 1 g/l of NaCl was the best for podophyllic acid production (i.e. 47.6 +/- 4.0 mg/l). Secondly, the production of podophyllic acid was significantly enhanced by fed-batch biotransformation. When each 100 mg/l of podophyllotoxin was added to the biotransformation system after 4, 10 and 25 h of culture, respectively, podophyllic acid concentration reached 99.9 +/- 12.3 mg/l, enhanced by 284% comparing to one-time addition (i.e. 26.0 +/- 2.1 mg/l). The fundamental information obtained in this study provides a simple and efficient way to produce podophyllic acid. (C) 2008 Elsevier Ltd. All rights reserved.
KeywordBiotransformation Process Optimization Podophyllotoxin Podophyllic Acid Fed-batch Biotransformation Pseudomonas Aeruginosa
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2008.10.044
Indexed BySCI
Language英语
WOS KeywordSPIN-LABELED DERIVATIVES ; TOLUENE HYDROXYLATION ; PUTIDA UV4 ; DISCOVERY ; SUBSTRATE ; SYSTEM
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000263018300027
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6689
Collection生化工程国家重点实验室
Corresponding AuthorTang, YJ
Affiliation1.Hubei Univ Technol, Coll Bioengn, Hubei Prov Key Lab Ind Microbiol, Wuhan 430068, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
3.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
Recommended Citation
GB/T 7714
Li, Yan,Li, Yuan-Yuan,Mi, Zhi-Yuan,et al. Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066, Part II: Process optimization[J]. BIORESOURCE TECHNOLOGY,2009,100(7):2271-2277.
APA Li, Yan,Li, Yuan-Yuan,Mi, Zhi-Yuan,Li, Dong-Sheng,Tang, Ya-Jie,&Tang, YJ.(2009).Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066, Part II: Process optimization.BIORESOURCE TECHNOLOGY,100(7),2271-2277.
MLA Li, Yan,et al."Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066, Part II: Process optimization".BIORESOURCE TECHNOLOGY 100.7(2009):2271-2277.
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