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pH-Sensitive Magnetic Ion Exchanger for Protein Separation
Yang, Liangrong; Guo, Chen; Chen, Shu; Wang, Feng; Wang, Jing; An, Zhentao; Liu, Chunzhao; Liu, Huizhou; Liu, CZ
2009-01-21
Source PublicationINDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
ISSN0888-5885
Volume48Issue:2Pages:944-950
AbstractA pH-sensitive magnetic ion exchanger was synthesized by binding carboxymethylated chitosan (CMCH) covalently on the surface of Fe(3)O(4) nanoparticles. The diameter for magnetic particles observed at 25 degrees C was 15 nm. The ion exchanger was superparamagnetic with a saturation magnetization of 64.21 emu/g and an isoelectric point (pI) of 5.75. In a model system, the laccase adsorption capacity reached equilibrium within 15 min (pH 5). The adsorption process followed the Langmuir adsorption isotherm, and the maximum equilibrium adsorption capacity was calculated to be 198.81 mg/g. The laccase can be completely desorbed at pH 8. About 97% laccase can be effectively desorbed from the surface of particles within 15 min. Moreover, the specific activity of the laccase remained constant during the adsorption and desorption process. Finally, the pH-sensitive magnetic ion exchanger was used for separation of laccase directly from culture supernatant, and nearly pure laccase was isolated by a single step with an activity recovery rate of 63%.
KeywordMetal Affinity Adsorption Trametes-versicolor Chitosan Laccase Purification Nanoparticles Particles Beads Capacity Chelator
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1021/ie800969q
Indexed BySCI
Language英语
WOS KeywordMETAL AFFINITY ADSORPTION ; TRAMETES-VERSICOLOR ; CHITOSAN ; LACCASE ; PURIFICATION ; NANOPARTICLES ; PARTICLES ; BEADS ; CAPACITY ; CHELATOR
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
WOS IDWOS:000262677900048
Citation statistics
Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6761
Collection生化工程国家重点实验室
Corresponding AuthorLiu, CZ
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Yang, Liangrong,Guo, Chen,Chen, Shu,et al. pH-Sensitive Magnetic Ion Exchanger for Protein Separation[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2009,48(2):944-950.
APA Yang, Liangrong.,Guo, Chen.,Chen, Shu.,Wang, Feng.,Wang, Jing.,...&Liu, CZ.(2009).pH-Sensitive Magnetic Ion Exchanger for Protein Separation.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,48(2),944-950.
MLA Yang, Liangrong,et al."pH-Sensitive Magnetic Ion Exchanger for Protein Separation".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 48.2(2009):944-950.
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