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Effect of membrane parameters on the size and uniformity in preparing agarose beads by premix membrane emulsification
Zhou, Qjng-Zhu; Ma, Guang-Hui; Su, Zhi-Guo; Ma, GH
2009-01-20
Source PublicationJOURNAL OF MEMBRANE SCIENCE
ISSN0376-7388
Volume326Issue:2Pages:694-700
AbstractAgarose microbeads were prepared by premix membrane emulsification with Shirasu-Porous; Glass (SPG) membrane and Polyethylene (PE) membrane. The effects of membrane parameters, including pore size, pore size distribution, contact angle between membrane surface and the water phase, shape of pore opening and membrane thickness on size and uniformity of agarose beads were investigated in this study. The results showed that pore size distribution and shape of pore opening did not affect the emulsification results apparently within a wide range in premix membrane emulsification, not as the result in general emulsification. The contact angle between the water phase and the membrane surface must be large enough to obtain uniform-sized agarose beads in both direct membrane emulsification and premix membrane emulsification. The results also showed that the membrane pore size and thickness affected the size distribution of emulsion. Thicker membrane resulted in more uniform and smaller emulsion when the number of pass through membrane is controlled. There was a linear relationship between the number average diameter of agarose beads and membranes pores size in premix membrane emulsification. Agarose beads with diameters from 3.06 to 9.02 mu m were prepared by controlling membranes pore size. The ratio of the number average diameter of agarose beads to membrane pore diameters was found to be 0.486. (C) 2008 Elsevier B.V. All rights reserved.
KeywordAgarose Beads Uniform-size Premix Membrane Emulsification Polyethylene
SubtypeArticle
WOS HeadingsScience & Technology ; Technology ; Physical Sciences
DOI10.1016/j.memsci.2008.11.012
Indexed BySCI
Language英语
WOS KeywordMULTIPLE EMULSIONS
WOS Research AreaEngineering ; Polymer Science
WOS SubjectEngineering, Chemical ; Polymer Science
WOS IDWOS:000263006500054
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6762
Collection生化工程国家重点实验室
Corresponding AuthorMa, GH
AffiliationChinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Qjng-Zhu,Ma, Guang-Hui,Su, Zhi-Guo,et al. Effect of membrane parameters on the size and uniformity in preparing agarose beads by premix membrane emulsification[J]. JOURNAL OF MEMBRANE SCIENCE,2009,326(2):694-700.
APA Zhou, Qjng-Zhu,Ma, Guang-Hui,Su, Zhi-Guo,&Ma, GH.(2009).Effect of membrane parameters on the size and uniformity in preparing agarose beads by premix membrane emulsification.JOURNAL OF MEMBRANE SCIENCE,326(2),694-700.
MLA Zhou, Qjng-Zhu,et al."Effect of membrane parameters on the size and uniformity in preparing agarose beads by premix membrane emulsification".JOURNAL OF MEMBRANE SCIENCE 326.2(2009):694-700.
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