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Elementary mode analysis and metabolic flux analysis of L-glutamate biosynthesis by Corynebacterium glutamicum
Chen, Ning1; Du, Jun1; Liu, Hui2; Xu, Qingyang1; Chen, N
2009
Source PublicationANNALS OF MICROBIOLOGY
ISSN1590-4261
Volume59Issue:2Pages:317-322
AbstractIn this study we applied elementary mode analysis and metabolic flux analysis to L-glutamate biosynthesis by Corynebacterium glutamicum GDK-9. The theoretically optimal flux distribution with the maximal L-glutamate yield and the actual flux distribution of L-glutamate production were determined, which suggested that phosphoenol-pyruvate (PEP), 2-oxoglutarate (2-OXO) and iso-citric acid (ICI) were the key nodes of L-glutamate biosynthesis. It was also indicated that the carbon-fixation reaction from CO(2) had great influence on the flux distribution of the key nodes. Besides, the biosynthesis of lactate could greatly decrease the L-glutamate yield. Therefore we further developed a rational dissolved oxygen control strategy to improve the L-glutamate production by strengthening the carbon-fixation reaction from CO2 and reducing the lactate production. Under the optimized fermentation conditions, a final L-glutamate concentration of 141 g/L was obtained and the carbon yield of L-glutamate on glucose was 81.7%.
KeywordCorynebacterium Glutamicum L-glutamate Metabolic Pathway Analysis Optimization
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS KeywordL-THREONINE PRODUCTION ; ESCHERICHIA-COLI ; CULTURE-CONDITIONS ; NETWORK ANALYSIS ; PATHWAY ANALYSIS ; OPTIMIZATION ; IMPROVEMENT ; BATCH ; OVERPRODUCTION ; FERMENTATION
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000267606200018
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6767
Collection多相复杂系统国家重点实验室
Corresponding AuthorChen, N
Affiliation1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Chen, Ning,Du, Jun,Liu, Hui,et al. Elementary mode analysis and metabolic flux analysis of L-glutamate biosynthesis by Corynebacterium glutamicum[J]. ANNALS OF MICROBIOLOGY,2009,59(2):317-322.
APA Chen, Ning,Du, Jun,Liu, Hui,Xu, Qingyang,&Chen, N.(2009).Elementary mode analysis and metabolic flux analysis of L-glutamate biosynthesis by Corynebacterium glutamicum.ANNALS OF MICROBIOLOGY,59(2),317-322.
MLA Chen, Ning,et al."Elementary mode analysis and metabolic flux analysis of L-glutamate biosynthesis by Corynebacterium glutamicum".ANNALS OF MICROBIOLOGY 59.2(2009):317-322.
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