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Solid Phase Pegylation of Hemoglobin
Suo, Xiaoyan; Zheng, Chunyang; Yu, Pengzhan; Lu, Xiuling; Ma, Guanghui; Su, Zhiguo; Su, ZG
2009
Source PublicationARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
ISSN1073-1199
Volume37Issue:4Pages:147-155
AbstractA solid phase conjugation process was developed for attachment of polyethylene glycol to hemoglobin molecule. Bovine hemoglobin was loaded onto an ion exchange chromatography column and adsorbed by the solid medium. Succinimidyl carbonate mPEG was introduced in the mobile phase after the adsorption. Pegylation took place between the hemoglobin on the solid phase, and the pegylation reagent in the liquid phase. A further elution was carried out to separate the pegylated and the unpegylated protein. Analysis by HPSEC, SDS-PAGE, and MALLS demonstrated that the fractions eluted from the solid phase contained well-defined components. Pegylated hemoglobin with one PEG chain was obtained with the yield of 75%, in comparison to the yield of 30% in the liquid phase pegylation. The P50 values of the mono-pegylated hemoglobin, prepared with SC-mPEG 5kDa, 10kDa and 20kDa, were 19.97, 20.23 and 20.54 mmHg, which were much closer to the value of red blood cells than that of pegylated hemoglobin prepared with the conventional method.
KeywordSolid Phase Polyethylene Glycol Hemoglobin Red Blood Cell Substitute
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1080/10731190903041188
Indexed BySCI
Language英语
WOS KeywordBOVINE SERUM-ALBUMIN ; POLYETHYLENE-GLYCOL ; COVALENT ATTACHMENT ; BLOOD ; PROTEINS ; SURFACE
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering ; Materials Science
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials
WOS IDWOS:000267835500001
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6768
Collection生化工程国家重点实验室
Corresponding AuthorSu, ZG
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Suo, Xiaoyan,Zheng, Chunyang,Yu, Pengzhan,et al. Solid Phase Pegylation of Hemoglobin[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,2009,37(4):147-155.
APA Suo, Xiaoyan.,Zheng, Chunyang.,Yu, Pengzhan.,Lu, Xiuling.,Ma, Guanghui.,...&Su, ZG.(2009).Solid Phase Pegylation of Hemoglobin.ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,37(4),147-155.
MLA Suo, Xiaoyan,et al."Solid Phase Pegylation of Hemoglobin".ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY 37.4(2009):147-155.
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