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Nanoparticle-supported multi-enzyme biocatalysis with in situ cofactor regeneration
Liu, Wenfang3,4; Zhang, Songping3; Wang, Ping1,2; Wang, P
2009
Source PublicationJOURNAL OF BIOTECHNOLOGY
ISSN0168-1656
Volume139Issue:1Pages:102-107
AbstractAlthough there have been a long history of studying and using immobilized enzymes. little has been reported regarding the nature of immobilized cofactors. Herein we report that cofactor NAD(H) covalently attached to silica nanoparticles successfully coordinated with particle-immobilized enzymes and enabled multistep biotransformations. Specifically, silica nanoparticle-attached glutamate dehydrogenase (GLDH), lactate dehydrogenase (LDH) and NAD(H) were prepared and applied to catalyze the coupled reactions for production of alpha-ketoglutarate and lactate with the cofactor regenerated within the reaction cycle. It appeared that particle-particle collision driven by Brownian motion of the nanoparticles provided effective interactions among the catalytic components, and thus realized a dynamic shuttling of the particle-supported cofactor between the two enzymes to keep the reaction cycles continuing. Total turnover numbers (TTNs) as high as 20,000 h(-1) were observed for the cofactor. It appeared to us that the use of particle-attached cofactor promises a new biochemical processing strategy for cofactor-dependent biotransformations. (c) 2008 Elsevier B.V. All rights reserved.
KeywordNanoparticles Enzyme Immobilization Multi-enzyme Biotransformation Cofactor Regeneration Bioprocessing Biosynthesis
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jbiotec.2008.09.015
Indexed BySCI
Language英语
WOS KeywordCHARGED MEMBRANE BIOREACTOR ; ALCOHOL-DEHYDROGENASE ; NADH REGENERATION ; NAD(P)-DEPENDENT OXIDOREDUCTASES ; SORBITOL PRODUCTION ; IMMOBILIZED NAD ; ENZYME REACTOR ; COENZYME ; RETENTION ; GLUTAMATE
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000262795600016
Citation statistics
Cited Times:58[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6787
Collection生化工程国家重点实验室
Corresponding AuthorWang, P
Affiliation1.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA
2.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
4.Beijing Inst Technol, Sch Chem Engn & Environm, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Liu, Wenfang,Zhang, Songping,Wang, Ping,et al. Nanoparticle-supported multi-enzyme biocatalysis with in situ cofactor regeneration[J]. JOURNAL OF BIOTECHNOLOGY,2009,139(1):102-107.
APA Liu, Wenfang,Zhang, Songping,Wang, Ping,&Wang, P.(2009).Nanoparticle-supported multi-enzyme biocatalysis with in situ cofactor regeneration.JOURNAL OF BIOTECHNOLOGY,139(1),102-107.
MLA Liu, Wenfang,et al."Nanoparticle-supported multi-enzyme biocatalysis with in situ cofactor regeneration".JOURNAL OF BIOTECHNOLOGY 139.1(2009):102-107.
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