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正相和反相柱层析组合分离纯化紫杉醇
张志强; 苏志国
2000
Source Publication生物工程学报
Issue01Pages:73-77
Abstract采用正相氧化铝柱层析和反相C18柱层析从东北红豆杉培养细胞浸提物中分离纯化了紫杉醇。优化了氧化铝柱层析和反相柱层析的操作条件。实验发现,经过氧化铝柱层析后,测得的紫杉醇量大大增加。经两步层析,使紫杉醇的含量从小于10%提高到95%,样品中微量杂质继以重结晶步骤除去,即可获得纯度超过98%的紫杉醇晶体。采用13CNMR对晶体分析,所得产物结构与文献上紫杉醇的结构一致。
Keyword紫杉醇 正相柱层析 分离纯化
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/7208
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
张志强,苏志国. 正相和反相柱层析组合分离纯化紫杉醇[J]. 生物工程学报,2000(01):73-77.
APA 张志强,&苏志国.(2000).正相和反相柱层析组合分离纯化紫杉醇.生物工程学报(01),73-77.
MLA 张志强,et al."正相和反相柱层析组合分离纯化紫杉醇".生物工程学报 .01(2000):73-77.
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