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昆虫细胞-杆状病毒表达系统表达尿激酶原
孙洪亮; 常韶华; 李佐虎
1999
Source Publication生物工程学报
Issue03Pages:99-103
Abstract

在转瓶和2L搅拌反应器中,利用重组杆状病毒AcNPV感染sf9昆虫细胞表达尿激酶原。在转瓶中,细胞接毒密度12×106/mL、MOI=30时,尿激酶原活性达到1065IU/mL。研究了尿激酶原表达过程中葡萄糖、乳酸的代谢变化。实验结果表明细胞状态对尿激酶原的表达水平有显著影响。

KeywordSf9昆虫细胞 杆状病毒 悬浮培养 尿激酶原
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/7403
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
孙洪亮,常韶华,李佐虎. 昆虫细胞-杆状病毒表达系统表达尿激酶原[J]. 生物工程学报,1999(03):99-103.
APA 孙洪亮,常韶华,&李佐虎.(1999).昆虫细胞-杆状病毒表达系统表达尿激酶原.生物工程学报(03),99-103.
MLA 孙洪亮,et al."昆虫细胞-杆状病毒表达系统表达尿激酶原".生物工程学报 .03(1999):99-103.
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