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Sf9细胞的培养工艺
王晓迟; 戚艺华; 欧阳藩
1998
Source Publication化工冶金
Issue03Pages:86-90
Abstract对Sf9细胞的静止培养工艺进行了研究.首先对Sf9细胞进行环境pH值由低到高的驯化,发现该细胞对于pH值变化的适应能力十分有限,在初始pH值为7.0的培养液中即不能生长,而且在细胞生长过程中培养液的pH值会略有升高.其次进行了培养液血清浓度由高到低的驯化,结果表明该细胞对于低血清环境的适应性很强,培养液的血清浓度降至2%时细胞仍可生长.最后,研究了初始接种密度对Sf9细胞生长的影响,发现在其传代过程中,104个/ml数量级是其生长的临界接种密度
KeywordSf9细胞 培养工艺 Ph值 血清浓度 接种密度
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/7529
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
王晓迟,戚艺华,欧阳藩. Sf9细胞的培养工艺[J]. 化工冶金,1998(03):86-90.
APA 王晓迟,戚艺华,&欧阳藩.(1998).Sf9细胞的培养工艺.化工冶金(03),86-90.
MLA 王晓迟,et al."Sf9细胞的培养工艺".化工冶金 .03(1998):86-90.
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