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昆虫细胞Sf9悬浮培养表达尿激酶原(pro-UK)
常韶华; 孙洪亮; 郭雁; 李佐虎
1998
Source Publication药物生物技术
Issue04Pages:207-209
Abstract研究了悬浮培养昆虫细胞 Sf9时感染指数 (MOI)、感染时间和培养基营养状况对重组蛋白激酶原 (pro- UK)表达的影响。发现在转瓶培养中采用 MOI3、感染对数中期细胞所产生的重组蛋白的量最高 ,酶活可达 42 0 U/ml。在反应器中采用同样感染条件 ,重组蛋白的表达量可达530 U/ml。在转瓶培养中采取感染后换液处理 ,重组蛋白的表达可提高至 61 0 U/ml
Keyword昆虫细胞 重组杆状病毒 悬浮培养 尿激酶原
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/7629
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
常韶华,孙洪亮,郭雁,等. 昆虫细胞Sf9悬浮培养表达尿激酶原(pro-UK)[J]. 药物生物技术,1998(04):207-209.
APA 常韶华,孙洪亮,郭雁,&李佐虎.(1998).昆虫细胞Sf9悬浮培养表达尿激酶原(pro-UK).药物生物技术(04),207-209.
MLA 常韶华,et al."昆虫细胞Sf9悬浮培养表达尿激酶原(pro-UK)".药物生物技术 .04(1998):207-209.
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