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火炬松成熟合子胚培养直接器官发生和植株再生
唐巍; 欧阳藩; 郭仲琛
1997
Source Publication云南植物研究
Issue03Pages:71-74
Abstract基因型Hb,Ma和Mc的火炬松成熟合子胚在附加1.0mg/LNAA,4.0mg/LBA,500mg/LLH和500mg/L谷氨酰胺的TE培养基上培养12周后,在子叶和胚轴部位形成不定芽原基。然后将合子胚转移到附加0.5mg/LNAA,0.5mg/LIBA,2mg/LBA,500mg/LLH和500mg/L谷氨酰胺的TE不定芽分化培养基上,6周后分化产生大量不定芽。3种基因型中,Hb的直接不定芽分化频率最高,达58.2%。直接不定芽经伸长培养6周后,转移到附加0.1mg/LIBA,1.0mg/LBA和0.5mg/LGA3的TE生根培养基上,6周后直接不定芽的最高生根频率达46%。
Keyword火炬松 直接器官发生 植株再生
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/7664
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
唐巍,欧阳藩,郭仲琛. 火炬松成熟合子胚培养直接器官发生和植株再生[J]. 云南植物研究,1997(03):71-74.
APA 唐巍,欧阳藩,&郭仲琛.(1997).火炬松成熟合子胚培养直接器官发生和植株再生.云南植物研究(03),71-74.
MLA 唐巍,et al."火炬松成熟合子胚培养直接器官发生和植株再生".云南植物研究 .03(1997):71-74.
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