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湿地松体细胞胚胎发生和植株再生
唐巍; 欧阳藩; 郭仲琛
1997
Source Publication植物资源与环境
Issue02Pages:9-12
Abstract以湿地松(PinuseliotiEngelm.)的未成熟合子胚为外植体,在附加8mg/L2,4D和4mg/LBA的LP培养基上诱导出胚性愈伤组织。在含1mg/L2,4D和05mg/LBA的培养基上保持并增殖。提高培养基的渗透压后,愈伤组织内形成大量的胚性胚柄细胞团和早期原胚。在附加4mg/LABA,75g/LPEG和5g/L活性炭的LP培养基上,早期原胚发育形成粗壮的子叶胚。在无激素LP培养基上,成熟的体细胞胚萌发并发育形成再生完整植株。体细胞胚转换成小植株的频率为156%。
Keyword湿地松 体细胞胚胎发生 植株再生
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/7665
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
唐巍,欧阳藩,郭仲琛. 湿地松体细胞胚胎发生和植株再生[J]. 植物资源与环境,1997(02):9-12.
APA 唐巍,欧阳藩,&郭仲琛.(1997).湿地松体细胞胚胎发生和植株再生.植物资源与环境(02),9-12.
MLA 唐巍,et al."湿地松体细胞胚胎发生和植株再生".植物资源与环境 .02(1997):9-12.
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