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Analysis of mixtures of fatty acids and fatty alcohols in fermentation broth
Alternative TitleJ. Chromatogr. A
Liu, Yilan1,2; Chen, Ting3; Yang, Maohua1; Wang, Caixia1,2; Huo, Weiyan4; Yan, Daojiang1,2; Chen, Jinjin1,2; Zhou, Jiemin1,2; Xing, Jianmin1
2014-01-03
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1323Issue:3Pages:66-72
AbstractMicrobial production of fatty acids and fatty alcohols has attracted increasing concerns because of energy crisis and environmental impact of fossil fuels. Therefore, simple and efficient methods for the extraction and quantification of these compounds become necessary. In this study, a high-performance liquid chromatography-refractive index detection (HPLC-RID) method was developed for the simultaneous quantification of fatty acids and fatty alcohols in these samples. The optimum chromatographic conditions are C18 column eluted with methanol:water:acetic acid (90:9.9:0.1, v/v/v); column temperature, 26 C; flow rate, 1.0 mL/min. Calibration curves of all selected analytes showed good linearity (r(2) >= 0.9989). The intra-day and inter-day relative standard deviations (RSDs) of the 10 compounds were less than 4.46% and 5.38%, respectively, which indicated that the method had good repeatability and precision. Besides, a method for simultaneous extraction of fatty acids and fatty alcohols from fermentation broth was optimized by orthogonal design. The optimal extraction conditions were as follows: solvent, ethyl acetate; solvent to sample ratio, 0.5:1; rotation speed, 2 min at 260 rpm; extraction temperature, 10 degrees C. This study provides simple and fast methods to simultaneously extract and quantify fatty acids and fatty alcohols for the first time. It will be useful for the study of microbial production of these products. (C) 2013 Elsevier B.V. All rights reserved.; Microbial production of fatty acids and fatty alcohols has attracted increasing concerns because of energy crisis and environmental impact of fossil fuels. Therefore, simple and efficient methods for the extraction and quantification of these compounds become necessary. In this study, a high-performance liquid chromatography-refractive index detection (HPLC-RID) method was developed for the simultaneous quantification of fatty acids and fatty alcohols in these samples. The optimum chromatographic conditions are C18 column eluted with methanol:water:acetic acid (90:9.9:0.1, v/v/v); column temperature, 26 C; flow rate, 1.0 mL/min. Calibration curves of all selected analytes showed good linearity (r(2) >= 0.9989). The intra-day and inter-day relative standard deviations (RSDs) of the 10 compounds were less than 4.46% and 5.38%, respectively, which indicated that the method had good repeatability and precision. Besides, a method for simultaneous extraction of fatty acids and fatty alcohols from fermentation broth was optimized by orthogonal design. The optimal extraction conditions were as follows: solvent, ethyl acetate; solvent to sample ratio, 0.5:1; rotation speed, 2 min at 260 rpm; extraction temperature, 10 degrees C. This study provides simple and fast methods to simultaneously extract and quantify fatty acids and fatty alcohols for the first time. It will be useful for the study of microbial production of these products. (C) 2013 Elsevier B.V. All rights reserved.
KeywordFatty Acids Fatty Alcohols Fermentation Broth Hplc-rid
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2013.10.094
URL查看原文
Indexed BySCI
Language英语
WOS KeywordIONIZATION MASS-SPECTROMETRY ; ESCHERICHIA-COLI ; METHYL-ESTERS ; EXTRACTION ; IDENTIFICATION ; PREDICTION ; CHEMICALS ; LEAVES ; FUELS
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000329953800008
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/8028
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Beijing Forestry Univ, Dept Biol Sci & Technol, Beijing 100083, Peoples R China
4.Huabei Oilfield 1 Middle Sch, Cangzhou City 062550, Hebei Province, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yilan,Chen, Ting,Yang, Maohua,et al. Analysis of mixtures of fatty acids and fatty alcohols in fermentation broth[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1323(3):66-72.
APA Liu, Yilan.,Chen, Ting.,Yang, Maohua.,Wang, Caixia.,Huo, Weiyan.,...&Xing, Jianmin.(2014).Analysis of mixtures of fatty acids and fatty alcohols in fermentation broth.JOURNAL OF CHROMATOGRAPHY A,1323(3),66-72.
MLA Liu, Yilan,et al."Analysis of mixtures of fatty acids and fatty alcohols in fermentation broth".JOURNAL OF CHROMATOGRAPHY A 1323.3(2014):66-72.
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