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beta-poly( L- malic acid) production by fed- batch culture of Aureobasidium pullulans ipe-1 with mixed sugars
Alternative TitleEng. Life Sci.
Cao, Weifeng1; Luo, Jianquan1,2; Qi, Benkun1; Zhao, Juan3; Qiao, Changsheng4; Ding, Luhui2; Su, Yi1; Wan, Yinhua1
2014-03-01
Source PublicationENGINEERING IN LIFE SCIENCES
ISSN1618-0240
Volume14Issue:2Pages:180-189
Abstract-poly(l-malic acid) (PMLA) is a biopolyester, which has attracted growing attention due to its potential applications in medicine and other industries. In this study, the biosynthetic pathway of PMLA and the fermentation strategies with mixed sugars were both investigated to enhance PMLA production by Aureobasidium pullulans ipe-1. Metabolic intermediates and inhibitors were used to study the biosynthetic pathway of PMLA. It showed that exogenous addition of l-malic acid, succinic acid, TFA, and avidin had negligible effect on PMLA production, while pyruvic acid and biotin were the inhibitors, indicating that PMLA biosynthesis was probably related to phosphoenolpyruvate via oxaloacetate catalyzed by phosphoenolpyruvate carboxylase. Sucrose was suitable for achieving the highest PMLA concentration, while fructose generated a higher yield of PMLA (PMLA produced per biomass). Furthermore, the fed-batch culture using fed solution with different sugar mixture for PMLA production was implemented. During the fed-batch culture with mixed solution, fructose could increase PMLA production. Compared with the batch culture, the feeding with mixed sugar (sucrose and glucose) increased PMLA concentration by 23.9%, up to 63.2 g/L, and the final volume of the broth was increased by 25%. These results provide a good reference for process development and optimization of PMLA production.; -poly(l-malic acid) (PMLA) is a biopolyester, which has attracted growing attention due to its potential applications in medicine and other industries. In this study, the biosynthetic pathway of PMLA and the fermentation strategies with mixed sugars were both investigated to enhance PMLA production by Aureobasidium pullulans ipe-1. Metabolic intermediates and inhibitors were used to study the biosynthetic pathway of PMLA. It showed that exogenous addition of l-malic acid, succinic acid, TFA, and avidin had negligible effect on PMLA production, while pyruvic acid and biotin were the inhibitors, indicating that PMLA biosynthesis was probably related to phosphoenolpyruvate via oxaloacetate catalyzed by phosphoenolpyruvate carboxylase. Sucrose was suitable for achieving the highest PMLA concentration, while fructose generated a higher yield of PMLA (PMLA produced per biomass). Furthermore, the fed-batch culture using fed solution with different sugar mixture for PMLA production was implemented. During the fed-batch culture with mixed solution, fructose could increase PMLA production. Compared with the batch culture, the feeding with mixed sugar (sucrose and glucose) increased PMLA concentration by 23.9%, up to 63.2 g/L, and the final volume of the broth was increased by 25%. These results provide a good reference for process development and optimization of PMLA production.
KeywordAureobasidium Pullulans -poly (Malic Acid) Carbon Source Fed-batch Culture Mixed Sugars
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1002/elsc.201200189
URL查看原文
Indexed BySCI
Language英语
WOS KeywordFRUCTOSE PHOSPHOTRANSFERASE SYSTEM ; PHYSARUM-POLYCEPHALUM ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; POLY(BETA-L-MALIC ACID) ; SALMONELLA-TYPHIMURIUM ; ESCHERICHIA-COLI ; POLY(MALIC ACID) ; POLYMALIC ACID ; CARBON-SOURCES ; DRUG-DELIVERY
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000332612300009
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/8035
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Technol Compiegne, Biol Engn Dept, TIMR, EA 4297, Compiegne, France
3.Tianjin Univ Sci & Technol, Res Ctr Modern Anal Technol, Tianjin, Peoples R China
4.Tianjin Univ Sci & Technol, Dept Bioengn, Tianjin, Peoples R China
Recommended Citation
GB/T 7714
Cao, Weifeng,Luo, Jianquan,Qi, Benkun,et al. beta-poly( L- malic acid) production by fed- batch culture of Aureobasidium pullulans ipe-1 with mixed sugars[J]. ENGINEERING IN LIFE SCIENCES,2014,14(2):180-189.
APA Cao, Weifeng.,Luo, Jianquan.,Qi, Benkun.,Zhao, Juan.,Qiao, Changsheng.,...&Wan, Yinhua.(2014).beta-poly( L- malic acid) production by fed- batch culture of Aureobasidium pullulans ipe-1 with mixed sugars.ENGINEERING IN LIFE SCIENCES,14(2),180-189.
MLA Cao, Weifeng,et al."beta-poly( L- malic acid) production by fed- batch culture of Aureobasidium pullulans ipe-1 with mixed sugars".ENGINEERING IN LIFE SCIENCES 14.2(2014):180-189.
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