CAS OpenIR  > 研究所(批量导入)
Bioinspired Approach to Multienzyme Cascade System Construction for Efficient Carbon Dioxide Reduction
Alternative TitleACS Catal.
Wang, Xiaoli1,2,3; Li, Zheng1,3; Shi, Jiafu1,3; Wu, Hong1,3; Jiang, Zhongyi1,2,3; Zhang, Wenyan1,3; Song, Xiaokai1,3; Ai, Qinghong1,3
2014-03-01
Source PublicationACS CATALYSIS
ISSN2155-5435
Volume4Issue:3Pages:962-972
AbstractAn efficient multienzyme cascade system based on ultrathin, hybrid microcapsules was constructed for converting CO2 to methanol by combining the unique functions of catechol and gelatin. Gelatin was modified with catechol groups (GelC) via well-defined EDC/NHS chemistry, thus endowed with the ability to covalently attach enzyme molecules. Next, the first enzyme (FateDH)-containing CaCO3 templates were synthesized via coprecipitation and coated with a GelC layer. Afterward, GelC was covalently attached with the second enzyme (FaldDH) via Michael addition and Schiff base reactions. Then, GelC induced the hydrolysis and condensation of silicate, and the third enzyme (YADH) was entrapped accompanying the formation of silica particles. After removal of CaCO3 templates, the GelCSi-based multienzyme system was obtained, in which the three enzymes were appropriately positioned in different places of the GelCSi microcapsules, and the amount of individual enzyme was regulated according to enzyme activity. The system exhibited high activity and stability for converting CO2 into methanol. In detail, the system displayed much higher methanol yield and selectivity (71.6%, 86.7%) than that of multienzyme in free form (35.5%, 47.3%). The methanol yield remained 52.6% after nine times of recycling. This study will provide some guidance on constructing diverse scaffolds for applications in catalysis, drug and gene delivery, and biosensors.; An efficient multienzyme cascade system based on ultrathin, hybrid microcapsules was constructed for converting CO2 to methanol by combining the unique functions of catechol and gelatin. Gelatin was modified with catechol groups (GelC) via well-defined EDC/NHS chemistry, thus endowed with the ability to covalently attach enzyme molecules. Next, the first enzyme (FateDH)-containing CaCO3 templates were synthesized via coprecipitation and coated with a GelC layer. Afterward, GelC was covalently attached with the second enzyme (FaldDH) via Michael addition and Schiff base reactions. Then, GelC induced the hydrolysis and condensation of silicate, and the third enzyme (YADH) was entrapped accompanying the formation of silica particles. After removal of CaCO3 templates, the GelCSi-based multienzyme system was obtained, in which the three enzymes were appropriately positioned in different places of the GelCSi microcapsules, and the amount of individual enzyme was regulated according to enzyme activity. The system exhibited high activity and stability for converting CO2 into methanol. In detail, the system displayed much higher methanol yield and selectivity (71.6%, 86.7%) than that of multienzyme in free form (35.5%, 47.3%). The methanol yield remained 52.6% after nine times of recycling. This study will provide some guidance on constructing diverse scaffolds for applications in catalysis, drug and gene delivery, and biosensors.
KeywordCo2 Conversion Multienzyme Cascade System Mussel-inspired Chemistry Biomimetic Mineralization Hybrid Microcapsules
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1021/cs401096c
URL查看原文
Indexed BySCI
Language英语
WOS KeywordRAY PHOTOELECTRON-SPECTROSCOPY ; GELATIN NANOPARTICLES ; ENZYME IMMOBILIZATION ; AMINO-ACIDS ; COATINGS ; CONVERSION ; HYDROGELS ; METHANOL ; CHEMISTRY ; MICROCAPSULES
WOS Research AreaChemistry
WOS SubjectChemistry, Physical
WOS IDWOS:000332756700028
Citation statistics
Cited Times:68[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/8041
Collection研究所(批量导入)
Affiliation1.Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Green Chem Technol, Tianjin 300072, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xiaoli,Li, Zheng,Shi, Jiafu,et al. Bioinspired Approach to Multienzyme Cascade System Construction for Efficient Carbon Dioxide Reduction[J]. ACS CATALYSIS,2014,4(3):962-972.
APA Wang, Xiaoli.,Li, Zheng.,Shi, Jiafu.,Wu, Hong.,Jiang, Zhongyi.,...&Ai, Qinghong.(2014).Bioinspired Approach to Multienzyme Cascade System Construction for Efficient Carbon Dioxide Reduction.ACS CATALYSIS,4(3),962-972.
MLA Wang, Xiaoli,et al."Bioinspired Approach to Multienzyme Cascade System Construction for Efficient Carbon Dioxide Reduction".ACS CATALYSIS 4.3(2014):962-972.
Files in This Item:
File Name/Size DocType Version Access License
Bioinspired Approach(3951KB) 限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Xiaoli]'s Articles
[Li, Zheng]'s Articles
[Shi, Jiafu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Xiaoli]'s Articles
[Li, Zheng]'s Articles
[Shi, Jiafu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Xiaoli]'s Articles
[Li, Zheng]'s Articles
[Shi, Jiafu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.