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Comparative analysis of anthocyanin biosynthesis during fruit development in two Lycium species
Alternative TitlePhysiol. Plant.
Zeng, Shaohua1; Wu, Min1; Zou, Caiyun1; Liu, Xiaomin1; Shen, Xiaofei2; Hayward, Alice1; Liu, Chunzhao3; Wang, Ying1,2
2014-04-01
Source PublicationPHYSIOLOGIA PLANTARUM
ISSN0031-9317
Volume150Issue:4Pages:505-516
AbstractDietary consumption of functional foods enriched in anthocyanins benefit for human health by protection against far-ranging human diseases. Delphinidin-derived anthocyanins (valuable as blue pigments and antioxidants) are accumulated specifically in the fruits of Lycium ruthenicum but not in the fruits of Lycium barbarum, suggesting the differences of anthocyanin biosynthesis between the two species. In this study, anthocyanin profiling confirmed that anthocyanins were increasingly accumulated during fruit ripening in L. ruthenicum, and sharply increased at full expanded mature fruit, while no anthocyanin were detected at any stage of L. barbarum fruit development. Several genes involved in anthocyanin biosynthesis were characterized in L. ruthenicum and L. barbarum fruits. Expression profiling of these genes during fruit development showed a significant positive correlation between transcript abundance and anthocyanin accumulation in L. ruthenicum fruit. Meanwhile, transcripts in L. barbarum fruit were either undetectable or were downregulated during fruit ripening, before increasing slightly in the final stages of maturation. In addition, the ratio of LrF3 ' 5H/LrF3 ' H transcription showed a gradual increase before 6 days after breaker (DAB) and a sharp enhancement at 10 DAB. Our results suggest that the expression patterns of both regulatory and structural genes and the transcriptional ratio of branch-node structural genes F3 ' 5 ' H/F3 ' H may determine the phenotypic difference in anthocyanin biosynthesis between L. ruthenicum and L. barbarum fruits.; Dietary consumption of functional foods enriched in anthocyanins benefit for human health by protection against far-ranging human diseases. Delphinidin-derived anthocyanins (valuable as blue pigments and antioxidants) are accumulated specifically in the fruits of Lycium ruthenicum but not in the fruits of Lycium barbarum, suggesting the differences of anthocyanin biosynthesis between the two species. In this study, anthocyanin profiling confirmed that anthocyanins were increasingly accumulated during fruit ripening in L. ruthenicum, and sharply increased at full expanded mature fruit, while no anthocyanin were detected at any stage of L. barbarum fruit development. Several genes involved in anthocyanin biosynthesis were characterized in L. ruthenicum and L. barbarum fruits. Expression profiling of these genes during fruit development showed a significant positive correlation between transcript abundance and anthocyanin accumulation in L. ruthenicum fruit. Meanwhile, transcripts in L. barbarum fruit were either undetectable or were downregulated during fruit ripening, before increasing slightly in the final stages of maturation. In addition, the ratio of LrF3 ' 5H/LrF3 ' H transcription showed a gradual increase before 6 days after breaker (DAB) and a sharp enhancement at 10 DAB. Our results suggest that the expression patterns of both regulatory and structural genes and the transcriptional ratio of branch-node structural genes F3 ' 5 ' H/F3 ' H may determine the phenotypic difference in anthocyanin biosynthesis between L. ruthenicum and L. barbarum fruits.
KeywordCell-suspension Cultures Flavonoid 3' Grape 5'-hydroxylase Genes Vitis-vinifera Transcription Factors Functional-analysis Negative Regulator Flower Color Expression 3'-hydroxylase Accumulation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1111/ppl.12131
URL查看原文
Indexed BySCI
Language英语
WOS KeywordFLAVONOID 3',5'-HYDROXYLASE GENES ; CELL-SUSPENSION CULTURES ; GRAPE VITIS-VINIFERA ; TRANSCRIPTION FACTORS ; FUNCTIONAL-ANALYSIS ; NEGATIVE REGULATOR ; FLOWER COLOR ; EXPRESSION ; 3'-HYDROXYLASE ; ACCUMULATION
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000332553600002
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/8056
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China
2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zeng, Shaohua,Wu, Min,Zou, Caiyun,et al. Comparative analysis of anthocyanin biosynthesis during fruit development in two Lycium species[J]. PHYSIOLOGIA PLANTARUM,2014,150(4):505-516.
APA Zeng, Shaohua.,Wu, Min.,Zou, Caiyun.,Liu, Xiaomin.,Shen, Xiaofei.,...&Wang, Ying.(2014).Comparative analysis of anthocyanin biosynthesis during fruit development in two Lycium species.PHYSIOLOGIA PLANTARUM,150(4),505-516.
MLA Zeng, Shaohua,et al."Comparative analysis of anthocyanin biosynthesis during fruit development in two Lycium species".PHYSIOLOGIA PLANTARUM 150.4(2014):505-516.
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