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Comparison of PLA Microparticles and Alum as Adjuvants for H5N1 Influenza Split Vaccine: Adjuvanticity Evaluation and Preliminary Action Mode Analysis
Alternative TitlePharm. Res.
Zhang, Weifeng1,2; Wang, Lianyan1; Liu, Yuan1,2; Chen, Xiaoming1,2; Li, Jiahui1; Yang, Tingyuan1; An, Wenqi3; Ma, Xiaowei3; Pan, Ruowen3; Ma, Guanghui1
2014-04-01
Source PublicationPHARMACEUTICAL RESEARCH
ISSN0724-8741
Volume31Issue:4Pages:1015-1031
AbstractTo compare the adjuvanticity of polymeric particles (new-generation adjuvant) and alum (the traditional and FDA-approved adjuvant) for H5N1 influenza split vaccine, and to investigate respective action mode. Vaccine formulations were prepared by incubating lyophilized poly(lactic acid) (PLA) microparticles or alum within antigen solution. Antigen-specific immune responses in mice were evaluated using ELISA, ELISpot, and flow cytometry assay. Adjuvants' action modes were investigated by determining antigen persistence at injection sites, local inflammation response, antigen transport into draining lymph node, and activation of DCs in secondary lymphoid organs (SLOs). Alum promoted antigen-specific humoral immune response. PLA microparticles augmented both humoral immune response and cell-mediated-immunity which might enhance cross-protection of influenza vaccine. With regard to action mode, alum adjuvant functions by improving antigen persistence at injection sites, inducing severe local inflammation, slightly improving antigen transport into draining lymph nodes, and improving the expression of MHC II on DCs in SLOs. PLA microparticles function by slightly improving antigen transport into draining lymph nodes, and promoting the expression of both MHC molecules and co-stimulatory molecules on DCs in SLOs. Considering the adjuvanticity and side effects (local inflammation) of both adjuvants, we conclude that PLA microparticles are promising alternative adjuvant for H5N1 influenza split vaccine.; To compare the adjuvanticity of polymeric particles (new-generation adjuvant) and alum (the traditional and FDA-approved adjuvant) for H5N1 influenza split vaccine, and to investigate respective action mode.
KeywordAdjuvanticity Alum Influenza Vaccine Microparticles Mode Of Action
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine
DOI10.1007/s11095-013-1224-z
URL查看原文
Indexed BySCI
Language英语
WOS KeywordVIRUS-LIKE PARTICLE ; IMMUNE-RESPONSES ; DELIVERY-SYSTEMS ; CELL DIFFERENTIATION ; CROSS-PRESENTATION ; DENDRITIC CELLS ; NANOPARTICLES ; ANTIGEN ; PROTECTION ; RECEPTORS
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Multidisciplinary ; Pharmacology & Pharmacy
WOS IDWOS:000333080100015
Citation statistics
Cited Times:31[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/8058
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Natl Key Lab Biochem Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Hualan Biol Engn Inc, Xinxiang 453003, Henan Province, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Weifeng,Wang, Lianyan,Liu, Yuan,et al. Comparison of PLA Microparticles and Alum as Adjuvants for H5N1 Influenza Split Vaccine: Adjuvanticity Evaluation and Preliminary Action Mode Analysis[J]. PHARMACEUTICAL RESEARCH,2014,31(4):1015-1031.
APA Zhang, Weifeng.,Wang, Lianyan.,Liu, Yuan.,Chen, Xiaoming.,Li, Jiahui.,...&Ma, Guanghui.(2014).Comparison of PLA Microparticles and Alum as Adjuvants for H5N1 Influenza Split Vaccine: Adjuvanticity Evaluation and Preliminary Action Mode Analysis.PHARMACEUTICAL RESEARCH,31(4),1015-1031.
MLA Zhang, Weifeng,et al."Comparison of PLA Microparticles and Alum as Adjuvants for H5N1 Influenza Split Vaccine: Adjuvanticity Evaluation and Preliminary Action Mode Analysis".PHARMACEUTICAL RESEARCH 31.4(2014):1015-1031.
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