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Multiscale evaluation of pore curvature effects on protein structure in nanopores
Alternative TitleJ. Mat. Chem. B
Hao, Dong-Xia1; Huang, Yong-Dong1; Wang, Kang2; Wei, Yu-Ping1; Zhou, Wei-Qing1; Li, Juan1; Ma, Guang-Hui1,3; Su, Zhi-Guo1,3
2014
Source PublicationJOURNAL OF MATERIALS CHEMISTRY B
ISSN2050-750X
Volume2Issue:13Pages:1770-1778
AbstractProtein structure in nanopores is an important determinant in porous substrate utilization in biotechnology and materials science. To date, accurate residue details of pore curvature induced protein binding and unfolding were still unknown. Here, a multiscale ensemble of chromatography, NMR hydrogen and deuterium (H/D) exchange, confocal scanning and molecular docking simulations was combined to obtain the protein adsorption information induced by pore size and curvature. Lysozyme and polystyrene microspheres within pores in the 14-120 nm range were utilized as models. With pore size increasing, the bound lysozyme presented a tendency of significantly decreased retention, less unfolding and fewer interacted sites. However, such a significant dependence between pore curvature and protein size only existed in a limited micro-pore range comparable to protein sizes. The mechanism behind the above events could be attributed to the diverse protein interaction area determined by pore curvature and size change, by models calculating the binding of lysozyme onto surfaces. Another surface of opposite curvature for nanoparticles was also calculated and compared, the rules were similar but with opposite direction and such a critical size also existed. These studies of proteins on curved interfaces may ultimately help to guide the design of novel porous materials and assist in the discrimination of the target protein from molecular banks.; Protein structure in nanopores is an important determinant in porous substrate utilization in biotechnology and materials science. To date, accurate residue details of pore curvature induced protein binding and unfolding were still unknown. Here, a multiscale ensemble of chromatography, NMR hydrogen and deuterium (H/D) exchange, confocal scanning and molecular docking simulations was combined to obtain the protein adsorption information induced by pore size and curvature. Lysozyme and polystyrene microspheres within pores in the 14-120 nm range were utilized as models. With pore size increasing, the bound lysozyme presented a tendency of significantly decreased retention, less unfolding and fewer interacted sites. However, such a significant dependence between pore curvature and protein size only existed in a limited micro-pore range comparable to protein sizes. The mechanism behind the above events could be attributed to the diverse protein interaction area determined by pore curvature and size change, by models calculating the binding of lysozyme onto surfaces. Another surface of opposite curvature for nanoparticles was also calculated and compared, the rules were similar but with opposite direction and such a critical size also existed. These studies of proteins on curved interfaces may ultimately help to guide the design of novel porous materials and assist in the discrimination of the target protein from molecular banks.
KeywordHydrophobic Interaction Chromatography Liquid-chromatography Silica Nanoparticles Porous Microspheres Globular-proteins Binding Behavior Adsorption Lysozyme Stability Size
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1039/c3tb21714k
URL查看原文
Indexed BySCI
Language英语
WOS KeywordHYDROPHOBIC INTERACTION CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; SILICA NANOPARTICLES ; POROUS MICROSPHERES ; GLOBULAR-PROTEINS ; BINDING BEHAVIOR ; ADSORPTION ; LYSOZYME ; STABILITY ; SIZE
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, Biomaterials
WOS IDWOS:000332481700003
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/8145
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Hebei Univ Technol, Chem Engn Sch, Tianjin 300130, Peoples R China
3.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China
Recommended Citation
GB/T 7714
Hao, Dong-Xia,Huang, Yong-Dong,Wang, Kang,et al. Multiscale evaluation of pore curvature effects on protein structure in nanopores[J]. JOURNAL OF MATERIALS CHEMISTRY B,2014,2(13):1770-1778.
APA Hao, Dong-Xia.,Huang, Yong-Dong.,Wang, Kang.,Wei, Yu-Ping.,Zhou, Wei-Qing.,...&Su, Zhi-Guo.(2014).Multiscale evaluation of pore curvature effects on protein structure in nanopores.JOURNAL OF MATERIALS CHEMISTRY B,2(13),1770-1778.
MLA Hao, Dong-Xia,et al."Multiscale evaluation of pore curvature effects on protein structure in nanopores".JOURNAL OF MATERIALS CHEMISTRY B 2.13(2014):1770-1778.
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