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Preparation of uniform and large sized agarose microspheres by an improved membrane emulsification technique
Alternative TitlePowder Technol.
Zhao, Xi1,2; Wu, Jie1; Gong, Fang-Ling1; Cui, Jin-Mei1; Janson, Jan-Christer3; Ma, Guang-Hui1; Su, Zhi-Guo1
2014-02-01
Source PublicationPOWDER TECHNOLOGY
ISSN0032-5910
Volume253Issue:1Pages:444-452
AbstractThe SPG (Shirasu porous-glass) membrane emulsification technique has been subject to much attention for the preparation of uniform emulsions. However, so far primarily used for the production of droplets with sizes below approximately 60 mu m. A production bottleneck occurred if the desired size was further increased, especially when highly viscous dispersed phases were involved. To this end, an improved membrane emulsification technique was proposed and has been applied to the preparation of large agarose microspheres, with a size of around 90 mu m and with a narrow size distribution. The effects of important emulsification parameters, including the pore size of the SPG membrane, the operating pressure, the stirring rate of the continuous phase, the composition of the continuous oil phase, and the concentration of agarose in the dispersed water phase, have been extensively studied. Under optimum conditions, uniform-size agarose microspheres with an average diameter of 93 pm and a size distribution index of 0.65 were successfully prepared. The average particle size of the home-made agarose microspheres was almost identical to that of the commercial product Sepharose 4 Fast Flow (4FF), which is produced by mechanical stirring and an additional sieving process. However, the size distribution of the former was much narrower than that of the latter. Therefore, the improved membrane emulsification technique presented here is promising for the application of high viscosity systems such as agarose solutions, and the production scale can be further enhanced by increasing the number of membrane units attached to the experimental apparatus. (C) 2013 Elsevier B.V. All rights reserved.; The SPG (Shirasu porous-glass) membrane emulsification technique has been subject to much attention for the preparation of uniform emulsions. However, so far primarily used for the production of droplets with sizes below approximately 60 mu m. A production bottleneck occurred if the desired size was further increased, especially when highly viscous dispersed phases were involved. To this end, an improved membrane emulsification technique was proposed and has been applied to the preparation of large agarose microspheres, with a size of around 90 mu m and with a narrow size distribution. The effects of important emulsification parameters, including the pore size of the SPG membrane, the operating pressure, the stirring rate of the continuous phase, the composition of the continuous oil phase, and the concentration of agarose in the dispersed water phase, have been extensively studied. Under optimum conditions, uniform-size agarose microspheres with an average diameter of 93 pm and a size distribution index of 0.65 were successfully prepared. The average particle size of the home-made agarose microspheres was almost identical to that of the commercial product Sepharose 4 Fast Flow (4FF), which is produced by mechanical stirring and an additional sieving process. However, the size distribution of the former was much narrower than that of the latter. Therefore, the improved membrane emulsification technique presented here is promising for the application of high viscosity systems such as agarose solutions, and the production scale can be further enhanced by increasing the number of membrane units attached to the experimental apparatus. (C) 2013 Elsevier B.V. All rights reserved.
KeywordSpg Membrane Emulsification Technique Agarose Microspheres Large Size Uniformity
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1016/j.powtec.2013.12.012
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPROCESS PARAMETERS ; MANUFACTURE ; DROPLETS ; BEADS ; GEL
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
WOS IDWOS:000332430600057
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/8175
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100190, Peoples R China
3.Uppsala Univ, Dept Phys & Analyt Chem, SE-75123 Uppsala, Sweden
Recommended Citation
GB/T 7714
Zhao, Xi,Wu, Jie,Gong, Fang-Ling,et al. Preparation of uniform and large sized agarose microspheres by an improved membrane emulsification technique[J]. POWDER TECHNOLOGY,2014,253(1):444-452.
APA Zhao, Xi.,Wu, Jie.,Gong, Fang-Ling.,Cui, Jin-Mei.,Janson, Jan-Christer.,...&Su, Zhi-Guo.(2014).Preparation of uniform and large sized agarose microspheres by an improved membrane emulsification technique.POWDER TECHNOLOGY,253(1),444-452.
MLA Zhao, Xi,et al."Preparation of uniform and large sized agarose microspheres by an improved membrane emulsification technique".POWDER TECHNOLOGY 253.1(2014):444-452.
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