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青蒿毛状根和丛生芽培养生产青蒿素的代谢调控及生物反应器技术
刘春朝
Subtype博士
Thesis Advisor欧阳藩
1998-03-01
Degree Grantor中国科学院研究生院
Abstract青蒿素是从青蒿中分离出的一种抗疟药,特别对脑型疟疾和抗氯喹性疟疾具有特效,已成为世界卫生组织推荐的产品。由于天然资源的匮乏、青蒿素含量随季节和环境的不稳定以及大量采集造成的生态环境破坏以及化学合成成本高、难度大。因此,通过植物组织培养生产青蒿素成为目前研究的热点。论文从工程角度探讨青蒿毛状根和丛生芽培养合成青蒿素的动力学过程以及理化因素的代谢调控,并利用新型的生物反应器系统进行青蒿毛状根和丛生芽的培养,为大规模培养青蒿植物组织生产青蒿提供工程基础依据。
Other AbstractArtemisinin is an anti-malarial medicine isolated from the wormwood Artemisia annua L, a traditional Chinese herb of the family Compositae. It has a marked activity against chloroquinine-resistant and chloroquinine-sensitive strains of Plasmodium falciparum and is useful in treatment of cerebral malaria. Present commercial sources of most artemisinin are from field-grown leaves and flowering tops of Artemisia annua L, which are subject to seasonal and somatic variation. The total organic synthesis has been established, but is very complicate, low yields, and economically unattractive. For these reasons, artemisinin production in vitro cultures should be a promising alternative. In biochemical engineering aspects, the metabolic regulation and kinetics of artemisinin the biosynthesis by hairy root cultures and shoot cultures of Artemisia annua L and the bioreactor technology of plant tissue culture were studied in this thesis in order to provide the basis of scale-up production of artemisinin of A. annua L tissue cultures. The study on the kinetics of growth, artemisinin accumulation and nutrient uptake of A. annua L hairy root cultures were carried out, which provide and basis of the metabolic regulation and scale-up production of artemisinin by A. annua L hairy root cultures. The effect of light irradiation and temperature on artemisinin accumulation in A. annua L hairy root cultures were studied. The bi-staged temperature culture for artemisinin production by hairy root cultures is put forward. These results provides key technical parameters for scale-up production of artemisinin. Due to the completely unknown biosynthesis of artemisinin, the fungal elicited technology, which is employed to improve artemisinin accumulation in A. annua L hairy root cultures, provides an effective alternative of metabolic regulation for scale-up production of artemisinin. The shoot cultures of A, annua L were established and the effects of various conditions on growth and artemisinin accumulation of shoot cultures were investigated. On the basis of growth characteristics of hairy roots and shoots, a modified inner-loop airlift bioreactor and a modified inner-loop ultrasonic mist bioreactor were chosen to cultivate hairy root cultures and shoots of A. annua L respectively. The inoculation for scale-up cultivation of plant organs in bioreactors were also investigated, several inoculating devices were invented to improve the distribution of plant organs among the layers in bioreactors. Bioreactor systems, which had been developed to used not only in hairy root culture and shoots of A. annua L but also in other plant organs, open up the way to a bright further for the development of large-scale bioreactor technology on plant organ cultivation.
Pages148
Language中文
Document Type学位论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/8333
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
刘春朝. 青蒿毛状根和丛生芽培养生产青蒿素的代谢调控及生物反应器技术[D]. 中国科学院研究生院,1998.
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