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Thesis Advisor欧阳藩
Degree Grantor中国科学院研究生院
Other AbstractArtemisinin is an anti-malarial medicine isolated from the wormwood Artemisia annua L, a traditional Chinese herb of the family Compositae. It has a marked activity against chloroquinine-resistant and chloroquinine-sensitive strains of Plasmodium falciparum and is useful in treatment of cerebral malaria. Present commercial sources of most artemisinin are from field-grown leaves and flowering tops of Artemisia annua L, which are subject to seasonal and somatic variation. The total organic synthesis has been established, but is very complicate, low yields, and economically unattractive. For these reasons, artemisinin production in vitro cultures should be a promising alternative. In biochemical engineering aspects, the metabolic regulation and kinetics of artemisinin the biosynthesis by hairy root cultures and shoot cultures of Artemisia annua L and the bioreactor technology of plant tissue culture were studied in this thesis in order to provide the basis of scale-up production of artemisinin of A. annua L tissue cultures. The study on the kinetics of growth, artemisinin accumulation and nutrient uptake of A. annua L hairy root cultures were carried out, which provide and basis of the metabolic regulation and scale-up production of artemisinin by A. annua L hairy root cultures. The effect of light irradiation and temperature on artemisinin accumulation in A. annua L hairy root cultures were studied. The bi-staged temperature culture for artemisinin production by hairy root cultures is put forward. These results provides key technical parameters for scale-up production of artemisinin. Due to the completely unknown biosynthesis of artemisinin, the fungal elicited technology, which is employed to improve artemisinin accumulation in A. annua L hairy root cultures, provides an effective alternative of metabolic regulation for scale-up production of artemisinin. The shoot cultures of A, annua L were established and the effects of various conditions on growth and artemisinin accumulation of shoot cultures were investigated. On the basis of growth characteristics of hairy roots and shoots, a modified inner-loop airlift bioreactor and a modified inner-loop ultrasonic mist bioreactor were chosen to cultivate hairy root cultures and shoots of A. annua L respectively. The inoculation for scale-up cultivation of plant organs in bioreactors were also investigated, several inoculating devices were invented to improve the distribution of plant organs among the layers in bioreactors. Bioreactor systems, which had been developed to used not only in hairy root culture and shoots of A. annua L but also in other plant organs, open up the way to a bright further for the development of large-scale bioreactor technology on plant organ cultivation.
Document Type学位论文
Recommended Citation
GB/T 7714
刘春朝. 青蒿毛状根和丛生芽培养生产青蒿素的代谢调控及生物反应器技术[D]. 中国科学院研究生院,1998.
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青蒿毛状根和丛生芽培养生产青蒿素的代谢调(9753KB) 限制开放CC BY-NC-SAApplication Full Text
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