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利用pH电极流加葡萄糖培养重组大肠杆菌生产人α-肿瘤坏死因子的研究
丛春水; 邓继先; 苏志国
1999
Source Publication生物工程学报
Issue01Pages:89-94
Abstract应用一种新型的pH电极流加葡萄糖方法,用于培养重组大肠杆菌生产人α肿瘤坏死因子。流加后培养液中菌体OD600达到90,而α肿瘤坏死因子的比活保持105±011×105u/mg。与指数流加方法比较,二者能达到的最大菌体密度相近,而利用pH电极流加葡萄糖更具有设备简单,操作方便的特点。
Keyword人α-肿瘤坏死因子 重组大肠杆菌 流加 Ph
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/8495
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
丛春水,邓继先,苏志国. 利用pH电极流加葡萄糖培养重组大肠杆菌生产人α-肿瘤坏死因子的研究[J]. 生物工程学报,1999(01):89-94.
APA 丛春水,邓继先,&苏志国.(1999).利用pH电极流加葡萄糖培养重组大肠杆菌生产人α-肿瘤坏死因子的研究.生物工程学报(01),89-94.
MLA 丛春水,et al."利用pH电极流加葡萄糖培养重组大肠杆菌生产人α-肿瘤坏死因子的研究".生物工程学报 .01(1999):89-94.
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