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芽孢杆菌β-淀粉酶基因在大肠杆菌中的克隆与表达
王为先; 张沁; 申同健
1993
Source Publication遗传学报
Issue04Pages:374-380
Abstract本工作采用新设计的营养缺陷型淘汰筛选法,从本实验室筛选的芽孢杆菌Bacillus R2中分离到能够水解生淀粉的β-淀粉酶基因。该基因所在的DNA片段为5.25kb,在大肠杆菌(E.coli)中的表达产物具有与供体菌相同的淀粉酶特性,实验室条件下酶产量为500IU/ml以上,RDA值为57%,并且可以全部分泌到培养基中。
KeywordΒ-淀粉酶 生淀粉 基因克隆 胞外分泌
Indexed ByCSCD
CSCD IDCSCD:218971
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/9327
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
王为先,张沁,申同健. 芽孢杆菌β-淀粉酶基因在大肠杆菌中的克隆与表达[J]. 遗传学报,1993(04):374-380.
APA 王为先,张沁,&申同健.(1993).芽孢杆菌β-淀粉酶基因在大肠杆菌中的克隆与表达.遗传学报(04),374-380.
MLA 王为先,et al."芽孢杆菌β-淀粉酶基因在大肠杆菌中的克隆与表达".遗传学报 .04(1993):374-380.
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