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长白山白眉蝮蛇毒类凝血酶Gussurobin基因的克隆、表达与纯化(英文)
杨青; 胡学军; 许小明; 安利佳; 袁晓东; 苏志国; JANSON Jan-Christer
2002
Source Publication生物化学与生物物理学报
Issue01Pages:6-10
Abstract根据同源性 ,在高度保守的上游信号肽区域设计引物 ,通过RT PCR反应 ,从长白山白眉蝮蛇 (Gloydiusussurensis)毒腺总RNA中克隆得到类凝血酶 gussurobincDNA ,双向测序得到 gussurobin基因的全序列并由此推测出相应的氨基酸序列。与其他已知的类凝血酶不同 ,gussurobin只含有一个可能的糖基化位点 ,即Asn12 4 Ser12 5 Thr12 6。将gussurobin基因克隆到表达载体 pPIC9K中 ,电极转化至毕氏酵母菌株GS115中 ,经G418抗性筛选和营养缺陷型筛选获得重组子。经摇瓶培养 ,获得表达。经过柱层析分离 ,获得SDS PAGE电泳纯的重组gussurobin。
Keyword类凝血酶 Gussurobin Cdna序列 表达 纯化
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/9492
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
杨青,胡学军,许小明,等. 长白山白眉蝮蛇毒类凝血酶Gussurobin基因的克隆、表达与纯化(英文)[J]. 生物化学与生物物理学报,2002(01):6-10.
APA 杨青.,胡学军.,许小明.,安利佳.,袁晓东.,...&JANSON Jan-Christer.(2002).长白山白眉蝮蛇毒类凝血酶Gussurobin基因的克隆、表达与纯化(英文).生物化学与生物物理学报(01),6-10.
MLA 杨青,et al."长白山白眉蝮蛇毒类凝血酶Gussurobin基因的克隆、表达与纯化(英文)".生物化学与生物物理学报 .01(2002):6-10.
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