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大连蛇岛蝮蛇类凝血酶基因克隆与表达研究
杨青; 雷旭宇; 许建强; 李民; 苏志国; 安利佳; 杨克瑞斯特·杨森
2003
Source Publication大连理工大学学报
Issue02Pages:156-159
Abstract根据同源性设计引物,通过RT-PCR方法从大连蛇岛蝮蛇毒腺总RNA中合成扩增出类凝血酶基因,并由此推测出其相应的氨基酸序列.将该基因克隆到表达载体pPIC9K中,经电激转化后整合至毕氏酵母细胞中获得表达.该表达产物经两步柱层析后,进行聚丙烯酰胺凝胶电泳,获得单一条带,并表现较强的生色底物活性.
Keyword基因表达/类凝血酶 克隆 纯化
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/9493
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
杨青,雷旭宇,许建强,等. 大连蛇岛蝮蛇类凝血酶基因克隆与表达研究[J]. 大连理工大学学报,2003(02):156-159.
APA 杨青.,雷旭宇.,许建强.,李民.,苏志国.,...&杨克瑞斯特·杨森.(2003).大连蛇岛蝮蛇类凝血酶基因克隆与表达研究.大连理工大学学报(02),156-159.
MLA 杨青,et al."大连蛇岛蝮蛇类凝血酶基因克隆与表达研究".大连理工大学学报 .02(2003):156-159.
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