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An effective immobilized haloalkane dehalogenase DhaA from Rhodococcus rhodochrous by adsorption, crosslink and PEGylation on meso-cellular foam 期刊论文
Authors:  Zheng, He;  Yu, Wei-Li;  Guo, Xuan;  Zhao, Yuan-Zhong;  Cui, Yan;  Hu, Tao;  Zhong, Jin-Yi
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Haloalkane dehalogenase  Meso-cellular foam  Enzyme immobilization  
Production of tartaric acid using immobilized recominant cis-epoxysuccinate hydrolase 期刊论文
BIOTECHNOLOGY LETTERS, 2017, 卷号: 39, 期号: 12, 页码: 1859-1863
Authors:  Wang, Ziqiang;  Su, Munan;  Li, Yanliang;  Wang, Yunshan;  Su, Zhiguo
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Affinity Immobilization  Enzymatic Hydrolysis  Cis-epoxysuccinate Hydrolase  L-(+)-tartaric Acid  
PEGylation with the thiosuccinimido butylamine linker significantly increases the stability of haloalkane dehalogenase DhaA 期刊论文
JOURNAL OF BIOTECHNOLOGY, 2017, 卷号: 254, 页码: 25-33
Authors:  Zhao, Yuan-Zhong;  Yu, Wei-Li;  Zheng, He;  Guo, Xuan;  Guo, Nan;  Hu, Tao;  Zhong, Jin-Yi
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Protein Stability  Pegylation  Halogenated Compound  Dhaa  Linker  
Improvement of the Production Efficiency of L-(+)-Tartaric Acid by Heterogeneous Whole-Cell Bioconversion 期刊论文
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2014, 卷号: 172, 期号: 8, 页码: 3989-4001
Authors:  Wang, Ziqiang;  Wang, Yunshan;  Shi, Hong;  Su, Zhiguo
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L-(+)-tartaric Acid  Cis-epoxysuccinate Hydrolase  Heterogeneous Bioconversion  Process Optimization  Scale-up  
环氧琥珀酸水解酶工程菌的构建及在L-(+)-酒石酸生产中的应用 学位论文
: 中国科学院研究生院, 2013
Authors:  王自强
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顺式环氧琥珀酸水解酶    l-(+)-酒石酸    基因克隆与表达    非均相酶促转化    金属螯合亲和固定化  
Purification and characterization of a cis-epoxysuccinic acid hydrolase from Nocardia tartaricans CAS-52, and expression in Escherichia coli 期刊论文
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2013, 卷号: 97, 期号: 6, 页码: 2433-2441
Authors:  Wang, Ziqiang;  Wang, Yunshan;  Su, Zhiguo
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Cis-epoxysuccinic Acid Hydrolase  Nocardia Tartaricans  Prokaryotic Expression  Enantioselectivity  
Expression and production of recombinant cis-epoxysuccinate hydrolase in Escherichia coli under the control of temperature-dependent promoter 期刊论文
JOURNAL OF BIOTECHNOLOGY, 2012, 卷号: 162, 期号: 2-3, 页码: 232-236
Authors:  Wang, Ziqiang;  Wang, Yunshan;  Shi, Hong;  Su, Zhiguo;  Wang, YS
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Cis-epoxysuccinate Hydrolase  Plpr Promoter  Prokaryotic Expression  Scale-up