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Preparation and structural regulation of macroporous agarose microspheres for highly efficient adsorption of giant biomolecules 期刊论文
COLLOID AND POLYMER SCIENCE, 2022, 卷号: 300, 期号: 6, 页码: 691-705
Authors:  Zhao, Lan;  Huang, Lan;  Huang, Yongdong;  Zhu, Kai;  Che, Xiangjing;  Du, Yuxiang;  Gao, Jingwei;  Hao, Dongxia;  Zhang, Rongyue;  Wang, Qibao;  Ma, Guanghui
Favorite  |  View/Download:33/0  |  Submit date:2023/02/24
Macroporous agarose microspheres  Micellar swelling method  Pore-forming  Chromatographic media  Adsorption  
Tailored nanodisc immobilization for one-step purification and reconstitution of cytochrome P450: A tool for membrane proteins' hard cases 期刊论文
JOURNAL OF SEPARATION SCIENCE, 2021, 页码: 12
Authors:  Zhao, Lan;  Tao, Jiaoli;  Huang, Yongdong;  Zhu, Kai;  Du, Yuxiang;  Hao, Dongxia;  Liu, Hongying;  Zhang, Rongyue;  Ma, Guanghui
Favorite  |  View/Download:85/0  |  Submit date:2021/08/31
cytochrome P450  membrane protein purification  nanodiscs  nanodisc-immobilized chromatography  reconstitution  
Facile one-pot emulsion/sol-gel method for preparing wrinkled silica microspheres 期刊论文
PARTICUOLOGY, 2021, 卷号: 56, 页码: 33-42
Authors:  Hu, Yuning;  Hao, Dongxia;  Gong, Fangling;  Gao, Yuanyuan;  Yan, Xingran;  Ma, Guanghui
Favorite  |  View/Download:76/0  |  Submit date:2021/08/31
Wrinkled silica microsphere  Membrane emulsification  Emulsion/sol-gel methoda  
Rapid and high-capacity loading of IgG monoclonal antibodies by polymer brush and peptides functionalized microspheres 期刊论文
JOURNAL OF CHROMATOGRAPHY A, 2021, 卷号: 1640, 页码: 10
Authors:  Hao, Dongxia;  Zhang, Rongyue;  Ge, Jia;  Ye, Peili;  Song, Cui;  Zhu, Kai;  Na, Xiangming;  Huang, Yongdong;  Zhao, Lan;  Zhou, Weiqing;  Su, Zhiguo;  Ma, Guanghui
Favorite  |  View/Download:69/0  |  Submit date:2021/08/31
Antibody purification  Peptide ligand  Polymer brush  Macroporous microspheres  
基于核磁共振饱和转移差谱技术筛选Protein A仿生多肽配基 期刊论文
生物加工过程, 2021, 卷号: 19, 期号: 01, 页码: 40-46
Authors:  王伟颖;  葛佳;  苏志国;  马光辉;  郑永祥;  郝冬霞;  余蓉
Favorite  |  View/Download:6/0  |  Submit date:2023/06/08
最小化配基  蛋白A  人抗体hIgG  理性设计  核磁共振饱和转移差谱  仿生多肽  
Manipulation of pore structure during manufacture of agarose microspheres for bioseparation 期刊论文
ENGINEERING IN LIFE SCIENCES, 2020, 页码: 10
Authors:  Zhao, Lan;  Huang, Yongdong;  Zhu, Kai;  Miao, Zhuang;  Zhao, Jiazhang;  Che, Xiang Jing;  Hao, Dongxia;  Zhang, Rongyue;  Ma, Guanghui
Favorite  |  View/Download:57/0  |  Submit date:2021/03/29
agarose microspheres  agarose types  crosslinking degree  gel filtration chromatography  pore structure  
用于单克隆抗体纯化的仿生多肽超大孔PGMA微球制备及其性能 期刊论文
过程工程学报, 2020, 卷号: 000, 期号: 1.0, 页码: 84
Authors:  葛佳;  那向明;  吴学星;  杨维兴;  郝冬霞;  马光辉
Favorite  |  View/Download:92/0  |  Submit date:2021/03/29
单克隆抗体  纯化  仿生多肽  超大孔  微球  
A minimalist peptide ligand for IgG by minimizing the binding domain of protein A 期刊论文
BIOCHEMICAL ENGINEERING JOURNAL, 2019, 卷号: 151, 页码: 8
Authors:  Wang, Weiying;  Hao, Dongxia;  Ge, Jia;  Zhao, Lan;  Huang, Yongdong;  Zhu, Kai;  Wu, Xvexing;  Su, Zhiguo;  Yu, Rong;  Ma, Guanghui
Adobe PDF(1375Kb)  |  Favorite  |  View/Download:65/0  |  Submit date:2020/05/06
Affinity Purification Chromatography  Minimalist Peptide  Protein a  Igg  Rational Design  
Fabrication of rigid and macroporous agarose microspheres by pre-cross-linking and surfactant micelles swelling method 期刊论文
COLLOIDS AND SURFACES B-BIOINTERFACES, 2019, 卷号: 182, 页码: 8
Authors:  Zhao, Xi;  Huang, Lan;  Wu, Jie;  Huang, Yong-Dong;  Zhao, Lan;  Wu, Nan;  Zhou, Wei-Qing;  Hao, Dong-Xia;  Ma, Guang-Hui;  Su, Zhi-Guo
Favorite  |  View/Download:72/0  |  Submit date:2019/12/02
Agarose microspheres  Pre-cross-linking  Surfactant micelles swelling method  Macropores  High-speed chromatography  
A novel rProtein A chromatographic media for enhancing cleaning-in-place performance 期刊论文
JOURNAL OF IMMUNOLOGICAL METHODS, 2018, 卷号: 460, 页码: 45-50
Authors:  Zhao, Lan;  Wei, Wei;  Huang, Yongdong;  Zhang, Rongyue;  Zhu, Kai;  Hao, Dongxia;  Su, Zhiguo;  Ma, Guanghui
Adobe PDF(837Kb)  |  Favorite  |  View/Download:67/0  |  Submit date:2018/10/11
Rprotein a Chromatographic Media  Alkali-resistant  Clean-in-place  Dynamic Binding Capacity  Mab Purification