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Prevention of aggregate formation through mechanism analysis in refolding of recombinant pertactin from Escherichia coli 期刊论文
PROCESS BIOCHEMISTRY, 2017, 卷号: 62, 期号: 00, 页码: 80-90
Authors:  Li, Zenglan;  Zhang, Chun;  Zhang, Yan;  Liu, Yongdong;  Li, Xiunan;  Ma, Guanghui;  Luo, Jim;  Su, Zhiguo
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Recombinant Pertactin  Refolding  Aggregates  Dilution  
High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration 期刊论文
PROTEIN EXPRESSION AND PURIFICATION, 2017, 卷号: 133, 页码: 152-159
Authors:  Wang, Qi;  Liu, Yongdong;  Zhang, Chun;  Guo, Fangxia;  Feng, Cui;  Li, Xiunan;  Shi, Hong;  Su, Zhiguo
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Rhcntf  High Hydrostatic Pressure  Inclusion Bodies  Hydrophobic Aggregation  Refolding  
Improving the refolding efficiency for proinsulin aspart inclusion body with optimized buffer compositions 期刊论文
PROTEIN EXPRESSION AND PURIFICATION, 2016, 卷号: 122, 期号: JUNE, 页码: 1-7
Authors:  Chen, Ying;  Wang, Qi;  Zhang, Chun;  Li, Xiunan;  Gao, Qiang;  Dong, Changqing;  Liu, Yongdong;  Su, Zhiguo
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Proinsulin  Inclusion Bodies  Additive  Oxidative Refolding  Diselenide  Aggregate  
Interaction of arginine with protein during refolding process probed by amide H/D exchange mass spectrometry and isothermal titration calorimetry 期刊论文
Biochimica Et Biophysica Acta-Proteins and Proteomics, 2015, 期号: 1, 页码: 39-45
Authors:  Zhao, D. W.;  Liu, Y. D.;  Zhang, G. F.;  Zhang, C.;  Li, X. A.;  Wang, Q. Q.;  Shi, H.;  Su, Z. G.
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Hdx-ms  Itc  Refolding  Arginine  Rhg-csf  Hydrogen/deuterium Exchange  Escherichia-coli  Hydrophobic Interaction  Disulfide-isomerase  Proposed Mechanism  Hydrogen-exchange  Renaturation  Aggregation  Lysozyme  Binding  
Membrane combined with hydrophilic macromolecules enhances protein refolding at high concentration 期刊论文
PROCESS BIOCHEMISTRY, 2014, 卷号: 49, 期号: 7, 页码: 1129-1134
Authors:  Zhao, Dawei;  Liu, Yongdong;  Wang, Yinjue;  Li, Xiunan;  Wang, Qingqing;  Su, Zhiguo
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Refolding  Ultrafiltration  Rhg-csf  Inclusion Bodies  Polyethylene Glycol  Dextran  
Construction, Expression and Refolding of a Bifunctional Fusion Protein Consisting of C-Terminal 12-Residue of Hirudin-PA and Reteplase 期刊论文
PROTEIN JOURNAL, 2012, 卷号: 31, 期号: 4, 页码: 328-336
Authors:  Gao, Ling;  Zhang, Chun;  Li, Lingling;  Liang, Lan;  Deng, Xuan;  Wu, Wutong;  Su, Zhiguo;  Yu, Rong;  Yu, R
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Bifunctional Fusion Protein  Hirudin-pa C-terminal 12-residue  Reteplase  Inclusion Body  Refolding  
Efficient preparation and PEGylation of recombinant human non-glycosylated erythropoietin expressed as inclusion body in E. coli 期刊论文
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2010, 卷号: 386, 期号: 1-2, 页码: 156-164
Authors:  Wang, Yin-Jue;  Liu, Yong-Dong;  Chen, Jing;  Hao, Su-Juan;  Hu, Tao;  Ma, Guang-Hui;  Su, Zhi-Guo;  Su, ZG
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Recombinant Erythropoietin  Non-glycosylated  Refolding  Purification  Pegylation  Half-life  
Glycerol-Assisted Hydrophobic Interaction Chromatography Improving Refolding of Recombinant Human Granulocyte Colony-Stimulating Factor 期刊论文
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2009, 卷号: 159, 期号: 3, 页码: 634-641
Authors:  Wang, Fangwei;  Liu, Yongdong;  Ma, Guanghui;  Su, Zhiguo
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Hydrophobic Interaction Chromatography  Refolding  Glycerol  Recombinant Human Granulocyte Colony-stimulating Factor  
Cooperative effects of urea and L-arginine on protein refolding 期刊论文
PROTEIN EXPRESSION AND PURIFICATION, 2009, 卷号: 66, 期号: 1, 页码: 82-90
Authors:  Chen, Jing;  Liu, Yongdong;  Li, Xiunan;  Wang, Yinjue;  Ding, Hong;  Ma, Guanghui;  Su, Zhiguo;  Su, ZG
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Refolding Kinetics  Urea  L-arginine  Guanidinium Chloride  Rhg-csf  
Immobilized Triton X-100-assisted refolding of Green Fluorescent Protein-Tobacco Etch Virus protease fusion protein using beta-cyclodextrin as the eluent 期刊论文
PROCESS BIOCHEMISTRY, 2009, 卷号: 44, 期号: 3, 页码: 277-282
Authors:  Li, Jing Jing;  Wang, Ai-Qing;  Janson, Jan-Christer;  Ballagi, Andras;  Chen, Jing;  Liu, Yong-Dong;  Ma, Guang-Hui;  Su, Zhi-Guo;  Su, ZG
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Immobilized Triton X-100  Sepharose High Performance  Refolding  Green Fluorescent Protein  Hydrophobic Interaction Chromatography  Artificial Chaperone  Beta-cyclodextrin